S77 plus Vitamins Medium

NaCl16.00 g
MgSO4·7H2O2.50 g
CaSO4·2H2O0.50 g
KCl0.40 g
Tris0.05 g
Glycine0.25 g
KNO30.10 g
K2HPO40.01 g
KBr32.50 mg
SrCl2·6H2O6.50 mg
AlCl4·6H2O250.00 g
RbCl100.00 g
LiCl H2O50.00 g
KI25.00 g
EDTANa250.00 mg
FeSO4·7H2O2.50 mg
MnSO4·4H2O203.00 g
ZnSO4·7H2O22.00 g
CuSO4·5H2O19.60 g
CoSO4·7H2O2.38 g
NaMoO4·2H2O1.26 g
Cyanocobalamin100.00 ng
Distilled water to1.0 L
Adjust pH to 4.0 - 5.0. Add 0.10g/L NaSiO3·5H2O while stirring continuously. Adjust pH to 8.0 when the silicate has dissolved.

S88 + vitamins:
Prepare as for
S77 + vitamins, but with the following modifications:

Omit Tris and replace with 0.50 g/L glycylglycine

Add 50.0 g/L thiamine HCl in addition to cyanocobalamin

Omit NaSiO3·5H2O and the associated prior step of adjusting the pH to 4.0 - 5.0

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