| NaCl | 16.00 g |
| MgSO4·7H2O | 2.50 g |
| CaSO4·2H2O | 0.50 g |
| KCl | 0.40 g |
| Tris | 0.05 g |
| Glycine | 0.25 g |
| KNO3 | 0.10 g |
| K2HPO4 | 0.01 g |
| KBr | 32.50 mg |
| SrCl2·6H2O | 6.50 mg |
| AlCl4·6H2O | 250.00 µg |
| RbCl | 100.00 µg |
| LiCl H2O | 50.00 µg |
| KI | 25.00 µg |
| EDTANa2 | 50.00 mg |
| FeSO4·7H2O | 2.50 mg |
| MnSO4·4H2O | 203.00 µg |
| ZnSO4·7H2O | 22.00 µg |
| CuSO4·5H2O | 19.60 µg |
| CoSO4·7H2O | 2.38 µg |
| NaMoO4·2H2O | 1.26 µg |
| Cyanocobalamin | 100.00 ng |
| Distilled water to | 1.0 L |
Omit Tris and replace with 0.50 g/L glycylglycine
Add 50.0 µg/L thiamine HCl in addition to cyanocobalamin
Omit NaSiO3·5H2O and the associated prior step of adjusting the pH to 4.0 - 5.0
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