f/2 Medium Derivatives
f/2-Si:
Prepare as for f/2 Medium but omit Na2SiO3·9H2O. Make final volume up to 1.0
L with filtered seawater. Autoclave after all additions.
f/2*:
Extra Silicon and Selenium in this medium are benificial to several diatom
strains. Prepare as for f/2 Medium, then add 1.0 ml of Selenium Stock
Solution (1.29 mg H2SeO3/L dH2O) and 1.0 ml of Silicate Stock Solution
(30.0 g Na2SiO3·9H2O/L dH2O). Make final volume up to 1.0 L with filtered seawater. Autoclave.
f/2+Org:
Dispense f/2 Medium into desired culture vessel, add Organics Stock Solution then autoclave. Organics Stock Solution should comprise 10% of the final volume of this medium.
Organics Stock Solution:
|
Sodium Acetate | 1.0 g |
|
Glucose | 6.0 g |
|
Sodium Succinate·6H2O | 3.0 g |
|
Neopeptone | 4.0 g |
|
Bacto Tryptone | 4.0 g |
|
Yeast extract | 2.0 g |
|
Distilled water to | 1.0 L |
Dispense in small aliquots and autoclave.
f/2 (35%SW):
This medium is used to maintain organisms from brackish water (approximately
21 ppt salinity). Mix 650 ml dH2O and 350 ml filtered seawater. Prepare f/2 Medium in the dilute seawater solution. Autoclave.
f/2-Si (75%SW):
This medium is used to maintain organisms from brackish waters
(approximately 24 ppt salinity). Mix 750 ml filtered seawater and 250 ml
dH2O. Prepare f/2-Si Medium in the dilute seawater solution. Autoclave.
f/2* (75%SW):
This medium is used to maintain diatoms from brackish waters (approximately
24 ppt salinity). Mix 750 ml filtered seawater and 250 ml dH2O. Prepare f/2* Medium in the dilute seawater solution. Autoclave.
f/4 (50%SW):
This medium is used to maintain organisms from brackish waters
(approximately 16 ppt salinity). Mix 500 ml f/2 Medium and 500 ml dH2O. Autoclave.
f/4-Si:
Add 500 ml f/2-Si Medium to 500 ml filtered seawater. Autoclave.
f/20-Si:
Add 100 ml f/2-Si Medium to 900 ml filtered seawater. Autoclave.
f/20-Si+EDTA:
Mix 100 mL f/2-Si Medium and 850 ml filtered seawater. Add 1.0 ml of 100mM EDTA Stock Solution (37.2g Na2EDTA 2H2O/L dH2O, pH 8). Bring up to 1.0 L with filtered seawater then autoclave.
f/50-Si:
This medium is a 1/25 dilution of f/2-Si Medium. To prepare, follow recipe
for f/2-Si, but use sterile 1/25 strength stock solutions. The dilute stock
solutions are added aseptically to autoclaved, filtered seawater.
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