S77 plus Vitamins Medium


NaCl 16.00 g
MgSO4 7H2O 2.50 g
CaSO4 2H2O 0.50 g
KCl 0.40 g
Tris 0.05 g
Glycine 0.25 g
KNO3 0.10 g
K2HPO4 0.01 g
KBr 32.50 mg
SrCl2 6H2O 6.50 mg
AlCl4 6H2O 250.00 µg
RbCl 100.00 µg
LiCl H2O 50.00 µg
KI 25.00 µg
EDTANa2 50.00 mg
FeSO4 7H2O 2.50 mg
MnSO4 4H2O 203.00 µg
ZnSO4 7H2O 22.00 µg
CuSO4 5H2O 19.60 µg
CoSO4 7H2O 2.38 µg
NaMoO4 2H2O 1.26 µg
Cyanocobalamin 100.00 ng
Distilled water to 1.0 L

Adjust pH to 4.0 - 5.0. Add 0.10g/L NaSiO3 5H2O while stirring continuously. Adjust pH to 8.0 when the silicate has dissolved.


S88 + vitamins:
Prepare as for
S77 + vitamins, but with the following modifications:

Omit Tris and replace with 0.50 g/L glycylglycine

Add 50.0 µg/L thiamine HCl in addition to cyanocobalamin

Omit NaSiO3 5H2O and the associated prior step of adjusting the pH to 4.0 - 5.0



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