ASW Medium - Artificial Seawater

"Ultramarine Synthetica"* sea salts 33.6 g
Extra salts stock solution 3.75 ml
Vitamin stock solution 2.5 ml
Soil extract (SE1) 25.0 ml
Tricine 0.50 g
Distilled water to 1.0 L

Adjust pH to 7.6 - 7.8 with 1M NaOH or HCl. Autoclave at 15 psi.

*Supplier: Waterlife Research Industries Ltd, 476 Bath Road, Longford, West Drayton, Middlesex UB7 0ED, UK. Tel (01753) 685696 Fax (01753) 685437

Extra salts stock solution:
NaNO3 30.0 g
Na2HPO4 1.2 g
K2HPO4 1.0 g
Distilled water to 1.0 L

Vitamin stock solution:
Biotin 0.0002 g
Calcium pantothenate 0.02 g
Cyanocobalamin 0.004 g
Folic acid 0.0004 g
Inositol 1.0 g
Nicotinic acid 0.02 g
Thiamine HCl 0.1 g
Thymine 0.6 g
Distilled water to 1.0 L

May be stored frozen at -20 C.

Soil Extract (SE1):
Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.

A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2-4 mm) or stored as it is prior to use.

Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No. 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (eg., 1 L or less for 15 minutes) and are then kept in a cool place (eg., a refrigerator) until required.

2ASW (double strength Artificial Seawater):
Follow the recipe for
ASW but add 35 g NaCl/L prior to autoclaving.

1:1 mixture of ASW and BG media.

Mix aseptically.

1:1 mixture of ASW and SES media.

Mix aseptically.

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