Dr. Michael Gilgan                              

Chemistry Section                           

Inspection Laboratory, DFO                 

P.O. Box 550/1721 Lower Water Street        

Halifax, Nova Scotia                        

B3J 2S7  Canada                             

902-426-6285         FAX: 902-426-5342              

Michael=W=Gilgan%INSP%DFOSF@sfnet.DFO.ca    





2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?



	I don't work with cyanotoxins but rather phycotoxins; toxins 

generated by marine algae.  The toxins don't kill plants generally, as the 

name implies, but rather kill animals.  For those not familiar with this 

field, these toxins include the paralytic shellfish poisons (PSP) and 

diarretic shellfish poisons (DSP), as well as others.  The shellfish merely 

are the accumulators of the toxins from the phytoplankton on which they feed.

	Since there is some overlap in toxins with those of cyanobacteria, 

and I am interested in toxins in general as well, I am quite interested in 

what will likely be discussed in Cyano-Tox.  I would not be surprised to find 

that the toxic cyanobacteria contribute significantly to shellfish toxicity 

in some locations.



3) What are some keywords that sum up your research involvements and 

interests?

	Involved with:

	Diarretic shellfish poisons (DSP)

	Domoic acid (ASP)

	              

	Have interests concerning:

	Paralytic shellfish poisons (PSP)

	Ciguatera toxins 

	Brevitoxins

	Toxic phytoplankton	

-----



Gary Jones

Principal Research Scientist

CSIRO

Division of Water Resources

Griffith, NSW 2680

Australia

Ph.   61-69-601-563

FAX  61-69-601-600

e-mail    gary@griffith.dwr.csiro.au



Research Interests:



I lead a project team looking at a range of subjects relating to the

genetics and biochemistry of toxin production and biodegradation, and

ecology of cyanobacterial blooms in Australian rivers and water storages.

We have also carried out work on activated carbon treatment for removal of

low level (<20 =B5g/L) microcystin in drinking water.



Following are some papers recently published or in press:



JONES G.J. and Orr P.T. (1993)  Release and degradation of microcystin

following algicide treatment of a Microcystis aeruginosa bloom in a

recreational lake, as determined by HPLC and protein phosphatase inhibition 

assay.  Water Research 28, 871-876



JONES GJ, Parker NS and Blackburn SI (1994)  A toxic bloom of Nodularia

spumigena Mertens in Orielton Lagoon, Tasmania.  Aust. J. Mar. Freshwatr. 45

(in press)



JONES. GJ, Falconer IF and Wilkins RM (1995)  Persistence of cyclic peptide

toxins in dried cyanobacterial crusts from Lake Mokoan, Australia.  

Environ. Toxicol. Water Qual. Vol. 20(1) (in press)



JONES GJ, Bourne  D, Blakeley R and Doelle H.  (1994)   Degradation of the

cyanobacterial hepatotoxin microcystin LR by aquatic bacteria.  Natural

Toxins (in press)



JONES G..J, Minato W, Craig K and Naylor R.  (1993)  The removal of low

level cyanobacterial peptide toxins from drinking water using powdered and

granular activated carbon and chlorine - results of laboratory and pilot

plant studies.  In Proc. 15th AWWA Federal Convention, 1993, Gold Coast, 

pp. 339-346



Matveev, V., Matveeva, L. and JONES G.J. (1994)  Phytoplankton stimulation

by mosquito fish in the presence of large Daphnia. Verhandlungen

derInternationalen Vereinigung fur Theoretische und Angewandte Limnologie. 

Vol. 25 (in press)



Matveev V, Matveeva L and JONES GJ (1994)  The ability of Daphnia carinata

King to control phytoplankton and resist cyanobacterial toxicity:

Implications for biomanipulation in Australia.  Aust. J. Mar. Freshwatr. 45 

(in press)



JONES, G.J. (1994) Weir pool conditions stimulating cyanobacterial blooms.

in "The Murrumbidgee River"  (eds. Roberts J. and Oliver, R.) (in press)



JONES GJ  (1994)  Bloom forming Blue-Green Algae (Cyanobacteria).  Chapter

in Aquatic Plants of Australia, G. Sainty and S. Jacobs (eds.) pp. 266-285





KEYWORDS for RESEARCH:



cyanobacteria, toxins, blooms, control, biodegradation, genetics, analysis,

water treatment, grazing, odours

---------



Philip Orr

CSIRO Division of Water Resources,

Locked Bag No3.                      Phone +61 69 62 5604 (Home)

Griffith NSW 2680                    Phone +61 69 60 1555 (Work)

Australia                            FAX   +61 69 60 1600 (Work)

philip@griffith.dwr.csiro.au



>2) What aspects of cyanotoxins do you work with (ie. ecological, 

toxicolocial, structure, etc.)?



Physiology of Toxin Production



>3) What are some keywords that sum up your research involvements and >   

interests?



Microcystin, growth, toxicity, nutrients



-------



Ben. M. Long

School of Botany

La Trobe University

Bundoora, Victoria, Australia

(Post Code 3083).



Phone: +61 03 9479 2771

Fax: +61 03 9479 1188



Research Topic;



Aspects of the biochemistry and genetics of microcystin biosynthesis in         

Microcystis aeruginosa.  I am a postgraduate research student working under    

the supervision of Dr. John W. Anderson.



Keywords;



Microcystin, Microcystis aeruginosa, Biosynthesis, Protein Phosphatases,

Cyanobacterial Culture, Molecular Biology.



--------



Anthony R. Arment

Dept. of Biological Sciences

Wright State University

Dayton, OH 45435

(513) 873-3173

aarment@desire.wright.edu



Research Topic:



	Identifying the microcystin synthetase genes and characterizing them. 

I am a graduate student under Dr. Wayne W. Carmichael.



Keywords:



microcystin, Microcystis aeruginosa, biosynthesis, molecular biology, shuttle

vectors, PCR



--



1) My email addresses:



a)  bfalch@pharma6.unizh.ch

b)  falch@ezinfo.vmsmail.ethz.ch





Beatrix Falch

ETH-Zurich

Department of Pharmacy

Winterthurerstr.190

CH-8057 Zurich



2) My studies concern the secondary metabolism of terrestrial and

freshwater cyanobacteria. My main interest is in isolating biologically

active compounds, such as antimicrobial, cytotoxic (antitumor), and

antiviral. Up to now I have isolated polyhalogenated phenols (ambigols A

and B, J. Org. Chem, 1993), glycolipids,  and hydrocarbons.



3) some keywords of my research:

bioassay guided isolation, structure elucidation, biologically active

compounds, ecological importance of secondary metabolites, investigation of

culture media of cultured cyanobacteria, chromatographic methods for

isolation



--



1) e-mail roglruiz@fox.cce.usp.br Rogerio Lacaz-Ruiz at FZEA/USP

CP23 Campus da USP-Pirassununga - SP Brazil CEP 13630-000

Departamento de Ciencias Basicas- Microbiologia



2) I work with new culture media for Spirulina spp, using a agroindustrial

residues as a source of mineral components.



3) Keywords...Spirulina/Culture media/residues/biomass production/microalgae/

applied research...





-----



(1) Address

Zeng Ming-Tao

Department of Biochemistry

Faculty of Life Sciences

Tel Aviv University

Ramat Aviv, Tel Aviv 69978

Israel



Tel: 00972-3-6409826

Fax: 00972-3-6415053



E-Mail: zeng10@ccsg.tau.ac.il



(2) Research Interests

Toxin from Synechocystis and other single cell cyanobacteria. Molecular 

biology aspects, Site-directed mutagenesis for toxin overproduction. 



(3) Keywords

Site-directed mutagenesis; Toxin overproduction. 



----

1) What is your full snail-mail address, including your name and

   department?



Christopher J. S. Bolch,

CSIRO Division of Fisheries,

GPO Box 1538,

Hobart, Tasmania,

Australia, 7001.



Australia

PH. 002 325314

FAX 002-325000



International

PH.  061-02-325314

FAX. 061-02-325000





2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?



Ecological, genetics.



3) What are some keywords that sum up your research involvements and interests?



Ecology, population genetics, isolation,laboratory culture, purification

(axenic cultures), laboratory studies, toxigenic cyanobacteria.



---



My name is Mary Allen, Department of Biological Sciences, Wellesley College,

Wellesley, MA 03181.



I work on cyanobacterial biochemistry in general, mostly nitrogen metabolism,

other than nitrogen fixation.



Key words:  biochemistry, nitrogen metabolism, anything on cyanobacteria.



---



1) What is your full snail-mail address, including your name and

   department?



Avigad Vonshak                 

Algal Biotechnology

J. Blaustein Institute for Desert Research

Ben Gurion University

Sede Boqer campus 84990

 Israel

     Telephone: 7-565825;       Facsimile:  7-555058;

     E-Mail address: avigad@BGUMAIL.BGU.AC.IL





2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?





I am not working on cyanotoxins yet interested in the potential of thos

toxines as high value products







3) What are some keywords that sum up your research involvements and interests?



Spirulina, Haematococcus, Biotechnology , Photoinhibition, Biomass



--



1. Dr. Anneliese Ernst

Fakultaet fuer Biologie

Universitaet Konstanz

Postfach 5560

D-78434 Konstanz

Germany



2. No actual work with cyanotoxins



3. nitrogen fixation, photosynthesis, respiration; 

nitrogenase, hydrogenase, unicellular and 

heterocyst-forming cyanobacteria.



--

Name: Dr. Donald J. Douglas

Address:    Institute for Marine Biosciences

            National Research Council, Canada

            1411 Oxford St., Halifax

            B3H 3Z1

            CANADA





Research Interests: Principally on toxins of marine eukaryotic algae (i.e.

domoic acid production by the marine pennate diatom, Pseudonitschia pungens).

 

    However, I have a research program on the culture of freshwater

cyanobacteria for investigation of production of both known and novel toxins.

 This includes work with Microcystis and Oscillatoria spp.  I also work with

several cultures of the marine cyanobacteria Synechococcus for research on

phycoerythrin pigments.  I work closely with analytical and biological

chemists using HPLC, Mass Spectrometry, and NMR on isolation/identification of

 toxins.



Key Words: freshwater/marine/cultures/toxins/chemistry/biosynthesis



--------





1) What is your full snail-mail address, including your name and

   department?

1.

Dr John Cobley

Dept of Chemistry

University of San Francisco

2130 Folton St

San Francisco CA94117



2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?



2.

cyanotoxin voyeur



3) What are some keywords that sum up your research involvements and interests?



3.

cyanobacteria

chromatic adaptation

molecular genetics



-------------------------



1 - My full snail-mail address:  Renato Molica  - Universidade Federal

do Rio de Janeiro - CCS - NPPN - Lab. de Cultivo e Fisiologia de Microal

gas - Ilha do Fundao - CEP 21941-590 - BRASIL  / E.mail: Rmolica@UFRJ.bitnet

or Rmolica@vm1.nce.ufrj.br



2 - What aspects of cyanotoxins do you work with? physiological/ecological



3 - Keywords: cyanobacteria/microcystin/light intensity/nutrients



--------

1.



	My address is as follows:

	Timothy I. Wood

	UMBC, Department of Biological Sciences

	5401 Wilkens Avenue

	Catonsville, MD  21228-5398

	phone:  410-455-2229



2.



I work in Dr. Richard Wolf's laboratory.  This is my first year in graduate 

school and we do not work with cyanotoxins.  However, we are studying the 

carbon metabolism of Synechococcus strains.  We are looking into gene 

regulation of the pentose phospate pathway enzymes.  Any information 

concerning Synechococcus R2 PCC7942 would greatly be appreciated.  Thank 

you very much. 



-------



Clive Ward

c/o Department of Biological Sciences

University of Dundee

Dundee,

DD1 4HN



(yes, it's Geoff Codd's lab)





I've just started my PhD, so it's all up in the air a bit at the 

moment, but generally speaking (as I understand it!) I will be 

looking into microbial degradation of microcystins, especially the 

variants LY, LW, and LF. Also comparing the toxicity and effects of 

these variants on various microbial components (ie. cilliates) of 

aquatic systems. 



I don't think I can say much more at the moment really.



Keywords: Microcystins  Degradation Toxicity



--------



1.  Name and address.  Dr. R.M. (Ray) Dawson, Aeronautical and Maritime 

Research Laboratory, GPO Box 4331, Melbourne VIC 3001, Australia.  Fax 

Int-61-3-626 8410.



2.  I have just commenced working in the area of toxinology, and am 

interested in mechanisms of action and research into possible antidotes.



3.  Keywords.  Chemical defence, mechanism, enzyme, inhibitor, inhalation 

toxicology



------------



Hello!

	My name is Steve Royal, and I am a Biotechnology honours student

at Flinders University in Adelaide, S.A. The project I am undertaking for

honours involves the investigation and (hopefully) identification of

bacteria involved in the biodegradation of nodularin. If succesful in

this, there is the scope for further research into developing bioreactors

for water supply detoxification.



My snail-mail address is:

	16 Ragless St.

	St. Marys

	S.A. 5042

	Australia.

	phone: +61-8-357 4191

	e-mail:royal@cleese.apana.org.au

I have only recently subscribed to cyano-tox, and I think that it is a

good idea. Hear from you soon!

				Steve Royal



----------





1) What is your full snail-mail address, including your name and

   department?



1) Timo Mikkola

     Helsinki University

     Department of Applied Microbiology

     Biocenter, Viikki

     00710 Helsinki





2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?



2) I'm studying ecological and physiological aspects for some 

     freshwater spieces.



3) What are some keywords that sum up your research involvements and interests?



3) Ecology, physiology, toxicology, systematics, anabaena, 

     microcystin, oscillatoria, nostoc etc.



-------



Thomas Rohrlack

Humboldt-University of Berlin

Department of Biology (Ecology)

Unter den Linden 6

D-10099 Berlin

Germany



e-mail: 101526.327@compuserve.com



Last week I joined your discussion group. Today I want to answer the 

questions of your e-mail.  I'm a doctorand at the Humboldt-University 

of Berlin. In course of this work I study the interactions between zooplankton 

(especially Daphnia) and different strains of Microcystis aeruginosa. 

I´d be very pleased if there are some colleagues with the same

research interests in your group.





keywords: Microcystis, Daphnia, toxic blue-greens



--------

My postal adress:



Ulrich Papke



Institut fuer Organische Chemie der Universitaet Hamburg



Martin-Luther-King-Platz 6



20146 Hamburg



Germany



I am interested in the chemical structures of metabolites of Cyanobacteria.

I am an organic chemist working in the field of natural products and

chemical ecology.



------



NAME,etc



Brenton C. Nicholson

Australian Water Quality Centre

Private Mail Bag 3

Salisbury, South Australia, 5108, AUSTRALIA



Phone: 61 8 259 0246



Fax: 61 8 259 0228



Email: brenton.nicholson@sawater.sa.gov.au



RESEARCH INTERESTS



Hepatotoxins, neurotoxins (especially PSPs recently discovered in

Australian Anabaena circinalis), their toxicity, methods for their analysis

(in particular in water), their effects on water quality (including risk

assessment to determine "safe" concentrations), their degradation and

removal in water treatment processes.



KEYWORDS



cyanobacterial toxins/ peptide hepatotoxins/ microcystins/ nodularin/

PSPs/ anatoxin/ phosphatase inhibition/ water quality/ water treatment



------



Martin Saker

James Cook University

Townsville 4811

Australia



Ph: +61 77 814311

Fax: +61 77 251570



Research interests: I am currently enrolled in a phd investigating causes 

of toxic cyanobacterial blooms in 2 drinking water supplies.



Keywords: Monitoring, Toxic blooms



-------



Anna Maria Sanangelantoni

Dipartimento di Genetica e Microbiologia

Via Abbiategrasso 207

27100 PAVIA

ITALY

Phone +39+382-505578

FAX +39+382-528496

E-mail SANAN@ipvgen.unipv.it



Research topics:



exactly the same of A.Arment : "identifying the microcystin sinthetase

genes...."

Same (or many?...) years ago I worked on Spirulina platensis

molecular biology with O.Ciferri O.Tiboni and G.Riccardi.

Then I switched to Archaebacteria. I have been coming back to cyano in

the last year.

Keywords:



Microcystis aeruginosa, Oscillatoria agardhii, mycrocystin synthetase

genes, molecular biology



---------



> 

> 1) What is your full snail-mail address, including your name and

>    department?



Katherine von Stackelberg

Menzie-Cura & Associates, Inc.

1 Courthouse Lane, Suite 2

Chelmsford, MA  01824

tel: 508-453-4300

fax: 508-453-7260



> 

> 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

>    structure, etc.)?



I do not work with cyanotoxins; I am a human health and ecological 

risk assessor and periodically we are asked to look into the issue of 

cyanobacteria. 

> 

> 3) What are some keywords that sum up your research involvements and interests?

> 

human health risk assessment

ecological risk assessment

ecotoxicology 



-----------



1. Address:

             Jeff Elhai

  Department of Biological Sciences          (Tel) 305-348-3584

  Florida International University           (Fax) 305-348-1986

       University Park Campus           (E-mail) Cyano@Servax.Fiu.Edu

       Miami FL 33199  U.S.A.



2. Aspects of cyanotoxins: I'm just a voyeur



3. Keywords: heterocyst, differentiation, pattern formation, Anabaena, Nostoc,

      symbiosis, cycad, plant-microbial interaction, gene regulation, cell

      cycle, DNA synthesis



---------



1) What is your full snail-mail address, including your name and

   department?



Professor Yasuhisa Asano

Biotechnology Research Center

Toyama Prefectural University

5180 Kurokawa, Kosugi, Toyama

JAPAN

Tel: +81-766-56-7500 ext 530

Fax: +81-766-56-2498

E-mail: asano@pu-toyama.ac.jp



2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

   structure, etc.)?



enzymology of peptide hydrolysis



3) What are some keywords that sum up your research involvements and interests?



enzymes

microorganisms

enzymatic synthesis

organic synthesis

D-amino acid

peptide



----



1) What is your full snail-mail address, including your name and

   department?



Synne Kleiven, Dep. of Economy, Environment and Sports, Telemark College,

3800 B=F8, Norway



2) What aspects of cyanotoxins do you work with (ie. ecological,=

 toxicolocial,

   structure, etc.)?=20



Ecological aspects



3) What are some keywords that sum up your research involvements and=

 interests?



Environmental factors, blooming, toxinproduction, oligotrophic lakes,

recreation, drinking water



------



Michael Herdman

Unité de Physiologie Microbienne, Institut Pasteur,

28 rue du Dr Roux, 75724 Paris cedex 15, France.

Tel: (33 1) 4568 8416

Fax: (33 1) 4061 3042

mherdman@pasteur.fr



Together with Rosi Rippka, I am responsible for the Pasteur Culture 

Collection of Cyanobacteria (PCC). Our catalogue lists about 450 strains of 

cyanobacteria in axenic culture, including a number of toxin-producing 

members of the genera Microcystis, Oscillatoria and Nodularia. Please 

contact me for further details of the Collection. Our work involves the 

general phenotypic and genotypic characterization of the strains, but we do 

not study toxins themselves.



Keywords: DNA base composition; DNA/DNA hybridization; genotype; 

heterotrophy; nitrogen fixation; phenotype; phylogeny; physiology; 

purification; 16S rRNA.



--



In response to your questions,  I lead a a group who are studying a range of

tropical and semi -tropical blue-green algae.  Our interests are driven by

the human health impacts of infestations of public water supplies in Australia.

>

>1) What is your full snail-mail address, including your name and

>   department?



Professor Michael R. Moore

National Research Centre for Environmental Toxicology

PO box 594, Coopers Plains, Brisbane, Queensland  4108  AUSTRALIA

>

>2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial,

>   structure, etc.)?

Human toxicology, structure of toxins, function and taxonomy

>

>3) What are some keywords that sum up your research involvements and interests?

Cylindrospermopsis, Toxins, Water supplies, Biomarkers, Interactions, Human

toxicology

>