Dr. Michael Gilgan Chemistry Section Inspection Laboratory, DFO P.O. Box 550/1721 Lower Water Street Halifax, Nova Scotia B3J 2S7 Canada 902-426-6285 FAX: 902-426-5342 Michael=W=Gilgan%INSP%DFOSF@sfnet.DFO.ca 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? I don't work with cyanotoxins but rather phycotoxins; toxins generated by marine algae. The toxins don't kill plants generally, as the name implies, but rather kill animals. For those not familiar with this field, these toxins include the paralytic shellfish poisons (PSP) and diarretic shellfish poisons (DSP), as well as others. The shellfish merely are the accumulators of the toxins from the phytoplankton on which they feed. Since there is some overlap in toxins with those of cyanobacteria, and I am interested in toxins in general as well, I am quite interested in what will likely be discussed in Cyano-Tox. I would not be surprised to find that the toxic cyanobacteria contribute significantly to shellfish toxicity in some locations. 3) What are some keywords that sum up your research involvements and interests? Involved with: Diarretic shellfish poisons (DSP) Domoic acid (ASP) Have interests concerning: Paralytic shellfish poisons (PSP) Ciguatera toxins Brevitoxins Toxic phytoplankton ----- Gary Jones Principal Research Scientist CSIRO Division of Water Resources Griffith, NSW 2680 Australia Ph. 61-69-601-563 FAX 61-69-601-600 e-mail gary@griffith.dwr.csiro.au Research Interests: I lead a project team looking at a range of subjects relating to the genetics and biochemistry of toxin production and biodegradation, and ecology of cyanobacterial blooms in Australian rivers and water storages. We have also carried out work on activated carbon treatment for removal of low level (<20 =B5g/L) microcystin in drinking water. Following are some papers recently published or in press: JONES G.J. and Orr P.T. (1993) Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay. Water Research 28, 871-876 JONES GJ, Parker NS and Blackburn SI (1994) A toxic bloom of Nodularia spumigena Mertens in Orielton Lagoon, Tasmania. Aust. J. Mar. Freshwatr. 45 (in press) JONES. GJ, Falconer IF and Wilkins RM (1995) Persistence of cyclic peptide toxins in dried cyanobacterial crusts from Lake Mokoan, Australia. Environ. Toxicol. Water Qual. Vol. 20(1) (in press) JONES GJ, Bourne D, Blakeley R and Doelle H. (1994) Degradation of the cyanobacterial hepatotoxin microcystin LR by aquatic bacteria. Natural Toxins (in press) JONES G..J, Minato W, Craig K and Naylor R. (1993) The removal of low level cyanobacterial peptide toxins from drinking water using powdered and granular activated carbon and chlorine - results of laboratory and pilot plant studies. In Proc. 15th AWWA Federal Convention, 1993, Gold Coast, pp. 339-346 Matveev, V., Matveeva, L. and JONES G.J. (1994) Phytoplankton stimulation by mosquito fish in the presence of large Daphnia. Verhandlungen derInternationalen Vereinigung fur Theoretische und Angewandte Limnologie. Vol. 25 (in press) Matveev V, Matveeva L and JONES GJ (1994) The ability of Daphnia carinata King to control phytoplankton and resist cyanobacterial toxicity: Implications for biomanipulation in Australia. Aust. J. Mar. Freshwatr. 45 (in press) JONES, G.J. (1994) Weir pool conditions stimulating cyanobacterial blooms. in "The Murrumbidgee River" (eds. Roberts J. and Oliver, R.) (in press) JONES GJ (1994) Bloom forming Blue-Green Algae (Cyanobacteria). Chapter in Aquatic Plants of Australia, G. Sainty and S. Jacobs (eds.) pp. 266-285 KEYWORDS for RESEARCH: cyanobacteria, toxins, blooms, control, biodegradation, genetics, analysis, water treatment, grazing, odours --------- Philip Orr CSIRO Division of Water Resources, Locked Bag No3. Phone +61 69 62 5604 (Home) Griffith NSW 2680 Phone +61 69 60 1555 (Work) Australia FAX +61 69 60 1600 (Work) philip@griffith.dwr.csiro.au >2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? Physiology of Toxin Production >3) What are some keywords that sum up your research involvements and > interests? Microcystin, growth, toxicity, nutrients ------- Ben. M. Long School of Botany La Trobe University Bundoora, Victoria, Australia (Post Code 3083). Phone: +61 03 9479 2771 Fax: +61 03 9479 1188 Research Topic; Aspects of the biochemistry and genetics of microcystin biosynthesis in Microcystis aeruginosa. I am a postgraduate research student working under the supervision of Dr. John W. Anderson. Keywords; Microcystin, Microcystis aeruginosa, Biosynthesis, Protein Phosphatases, Cyanobacterial Culture, Molecular Biology. -------- Anthony R. Arment Dept. of Biological Sciences Wright State University Dayton, OH 45435 (513) 873-3173 aarment@desire.wright.edu Research Topic: Identifying the microcystin synthetase genes and characterizing them. I am a graduate student under Dr. Wayne W. Carmichael. Keywords: microcystin, Microcystis aeruginosa, biosynthesis, molecular biology, shuttle vectors, PCR -- 1) My email addresses: a) bfalch@pharma6.unizh.ch b) falch@ezinfo.vmsmail.ethz.ch Beatrix Falch ETH-Zurich Department of Pharmacy Winterthurerstr.190 CH-8057 Zurich 2) My studies concern the secondary metabolism of terrestrial and freshwater cyanobacteria. My main interest is in isolating biologically active compounds, such as antimicrobial, cytotoxic (antitumor), and antiviral. Up to now I have isolated polyhalogenated phenols (ambigols A and B, J. Org. Chem, 1993), glycolipids, and hydrocarbons. 3) some keywords of my research: bioassay guided isolation, structure elucidation, biologically active compounds, ecological importance of secondary metabolites, investigation of culture media of cultured cyanobacteria, chromatographic methods for isolation -- 1) e-mail roglruiz@fox.cce.usp.br Rogerio Lacaz-Ruiz at FZEA/USP CP23 Campus da USP-Pirassununga - SP Brazil CEP 13630-000 Departamento de Ciencias Basicas- Microbiologia 2) I work with new culture media for Spirulina spp, using a agroindustrial residues as a source of mineral components. 3) Keywords...Spirulina/Culture media/residues/biomass production/microalgae/ applied research... ----- (1) Address Zeng Ming-Tao Department of Biochemistry Faculty of Life Sciences Tel Aviv University Ramat Aviv, Tel Aviv 69978 Israel Tel: 00972-3-6409826 Fax: 00972-3-6415053 E-Mail: zeng10@ccsg.tau.ac.il (2) Research Interests Toxin from Synechocystis and other single cell cyanobacteria. Molecular biology aspects, Site-directed mutagenesis for toxin overproduction. (3) Keywords Site-directed mutagenesis; Toxin overproduction. ---- 1) What is your full snail-mail address, including your name and department? Christopher J. S. Bolch, CSIRO Division of Fisheries, GPO Box 1538, Hobart, Tasmania, Australia, 7001. Australia PH. 002 325314 FAX 002-325000 International PH. 061-02-325314 FAX. 061-02-325000 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? Ecological, genetics. 3) What are some keywords that sum up your research involvements and interests? Ecology, population genetics, isolation,laboratory culture, purification (axenic cultures), laboratory studies, toxigenic cyanobacteria. --- My name is Mary Allen, Department of Biological Sciences, Wellesley College, Wellesley, MA 03181. I work on cyanobacterial biochemistry in general, mostly nitrogen metabolism, other than nitrogen fixation. Key words: biochemistry, nitrogen metabolism, anything on cyanobacteria. --- 1) What is your full snail-mail address, including your name and department? Avigad Vonshak Algal Biotechnology J. Blaustein Institute for Desert Research Ben Gurion University Sede Boqer campus 84990 Israel Telephone: 7-565825; Facsimile: 7-555058; E-Mail address: avigad@BGUMAIL.BGU.AC.IL 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? I am not working on cyanotoxins yet interested in the potential of thos toxines as high value products 3) What are some keywords that sum up your research involvements and interests? Spirulina, Haematococcus, Biotechnology , Photoinhibition, Biomass -- 1. Dr. Anneliese Ernst Fakultaet fuer Biologie Universitaet Konstanz Postfach 5560 D-78434 Konstanz Germany 2. No actual work with cyanotoxins 3. nitrogen fixation, photosynthesis, respiration; nitrogenase, hydrogenase, unicellular and heterocyst-forming cyanobacteria. -- Name: Dr. Donald J. Douglas Address: Institute for Marine Biosciences National Research Council, Canada 1411 Oxford St., Halifax B3H 3Z1 CANADA Research Interests: Principally on toxins of marine eukaryotic algae (i.e. domoic acid production by the marine pennate diatom, Pseudonitschia pungens). However, I have a research program on the culture of freshwater cyanobacteria for investigation of production of both known and novel toxins. This includes work with Microcystis and Oscillatoria spp. I also work with several cultures of the marine cyanobacteria Synechococcus for research on phycoerythrin pigments. I work closely with analytical and biological chemists using HPLC, Mass Spectrometry, and NMR on isolation/identification of toxins. Key Words: freshwater/marine/cultures/toxins/chemistry/biosynthesis -------- 1) What is your full snail-mail address, including your name and department? 1. Dr John Cobley Dept of Chemistry University of San Francisco 2130 Folton St San Francisco CA94117 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? 2. cyanotoxin voyeur 3) What are some keywords that sum up your research involvements and interests? 3. cyanobacteria chromatic adaptation molecular genetics ------------------------- 1 - My full snail-mail address: Renato Molica - Universidade Federal do Rio de Janeiro - CCS - NPPN - Lab. de Cultivo e Fisiologia de Microal gas - Ilha do Fundao - CEP 21941-590 - BRASIL / E.mail: Rmolica@UFRJ.bitnet or Rmolica@vm1.nce.ufrj.br 2 - What aspects of cyanotoxins do you work with? physiological/ecological 3 - Keywords: cyanobacteria/microcystin/light intensity/nutrients -------- 1. My address is as follows: Timothy I. Wood UMBC, Department of Biological Sciences 5401 Wilkens Avenue Catonsville, MD 21228-5398 phone: 410-455-2229 2. I work in Dr. Richard Wolf's laboratory. This is my first year in graduate school and we do not work with cyanotoxins. However, we are studying the carbon metabolism of Synechococcus strains. We are looking into gene regulation of the pentose phospate pathway enzymes. Any information concerning Synechococcus R2 PCC7942 would greatly be appreciated. Thank you very much. ------- Clive Ward c/o Department of Biological Sciences University of Dundee Dundee, DD1 4HN (yes, it's Geoff Codd's lab) I've just started my PhD, so it's all up in the air a bit at the moment, but generally speaking (as I understand it!) I will be looking into microbial degradation of microcystins, especially the variants LY, LW, and LF. Also comparing the toxicity and effects of these variants on various microbial components (ie. cilliates) of aquatic systems. I don't think I can say much more at the moment really. Keywords: Microcystins Degradation Toxicity -------- 1. Name and address. Dr. R.M. (Ray) Dawson, Aeronautical and Maritime Research Laboratory, GPO Box 4331, Melbourne VIC 3001, Australia. Fax Int-61-3-626 8410. 2. I have just commenced working in the area of toxinology, and am interested in mechanisms of action and research into possible antidotes. 3. Keywords. Chemical defence, mechanism, enzyme, inhibitor, inhalation toxicology ------------ Hello! My name is Steve Royal, and I am a Biotechnology honours student at Flinders University in Adelaide, S.A. The project I am undertaking for honours involves the investigation and (hopefully) identification of bacteria involved in the biodegradation of nodularin. If succesful in this, there is the scope for further research into developing bioreactors for water supply detoxification. My snail-mail address is: 16 Ragless St. St. Marys S.A. 5042 Australia. phone: +61-8-357 4191 e-mail:royal@cleese.apana.org.au I have only recently subscribed to cyano-tox, and I think that it is a good idea. Hear from you soon! Steve Royal ---------- 1) What is your full snail-mail address, including your name and department? 1) Timo Mikkola Helsinki University Department of Applied Microbiology Biocenter, Viikki 00710 Helsinki 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? 2) I'm studying ecological and physiological aspects for some freshwater spieces. 3) What are some keywords that sum up your research involvements and interests? 3) Ecology, physiology, toxicology, systematics, anabaena, microcystin, oscillatoria, nostoc etc. ------- Thomas Rohrlack Humboldt-University of Berlin Department of Biology (Ecology) Unter den Linden 6 D-10099 Berlin Germany e-mail: 101526.327@compuserve.com Last week I joined your discussion group. Today I want to answer the questions of your e-mail. I'm a doctorand at the Humboldt-University of Berlin. In course of this work I study the interactions between zooplankton (especially Daphnia) and different strains of Microcystis aeruginosa. I´d be very pleased if there are some colleagues with the same research interests in your group. keywords: Microcystis, Daphnia, toxic blue-greens -------- My postal adress: Ulrich Papke Institut fuer Organische Chemie der Universitaet Hamburg Martin-Luther-King-Platz 6 20146 Hamburg Germany I am interested in the chemical structures of metabolites of Cyanobacteria. I am an organic chemist working in the field of natural products and chemical ecology. ------ NAME,etc Brenton C. Nicholson Australian Water Quality Centre Private Mail Bag 3 Salisbury, South Australia, 5108, AUSTRALIA Phone: 61 8 259 0246 Fax: 61 8 259 0228 Email: brenton.nicholson@sawater.sa.gov.au RESEARCH INTERESTS Hepatotoxins, neurotoxins (especially PSPs recently discovered in Australian Anabaena circinalis), their toxicity, methods for their analysis (in particular in water), their effects on water quality (including risk assessment to determine "safe" concentrations), their degradation and removal in water treatment processes. KEYWORDS cyanobacterial toxins/ peptide hepatotoxins/ microcystins/ nodularin/ PSPs/ anatoxin/ phosphatase inhibition/ water quality/ water treatment ------ Martin Saker James Cook University Townsville 4811 Australia Ph: +61 77 814311 Fax: +61 77 251570 Research interests: I am currently enrolled in a phd investigating causes of toxic cyanobacterial blooms in 2 drinking water supplies. Keywords: Monitoring, Toxic blooms ------- Anna Maria Sanangelantoni Dipartimento di Genetica e Microbiologia Via Abbiategrasso 207 27100 PAVIA ITALY Phone +39+382-505578 FAX +39+382-528496 E-mail SANAN@ipvgen.unipv.it Research topics: exactly the same of A.Arment : "identifying the microcystin sinthetase genes...." Same (or many?...) years ago I worked on Spirulina platensis molecular biology with O.Ciferri O.Tiboni and G.Riccardi. Then I switched to Archaebacteria. I have been coming back to cyano in the last year. Keywords: Microcystis aeruginosa, Oscillatoria agardhii, mycrocystin synthetase genes, molecular biology --------- > > 1) What is your full snail-mail address, including your name and > department? Katherine von Stackelberg Menzie-Cura & Associates, Inc. 1 Courthouse Lane, Suite 2 Chelmsford, MA 01824 tel: 508-453-4300 fax: 508-453-7260 > > 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, > structure, etc.)? I do not work with cyanotoxins; I am a human health and ecological risk assessor and periodically we are asked to look into the issue of cyanobacteria. > > 3) What are some keywords that sum up your research involvements and interests? > human health risk assessment ecological risk assessment ecotoxicology ----------- 1. Address: Jeff Elhai Department of Biological Sciences (Tel) 305-348-3584 Florida International University (Fax) 305-348-1986 University Park Campus (E-mail) Cyano@Servax.Fiu.Edu Miami FL 33199 U.S.A. 2. Aspects of cyanotoxins: I'm just a voyeur 3. Keywords: heterocyst, differentiation, pattern formation, Anabaena, Nostoc, symbiosis, cycad, plant-microbial interaction, gene regulation, cell cycle, DNA synthesis --------- 1) What is your full snail-mail address, including your name and department? Professor Yasuhisa Asano Biotechnology Research Center Toyama Prefectural University 5180 Kurokawa, Kosugi, Toyama JAPAN Tel: +81-766-56-7500 ext 530 Fax: +81-766-56-2498 E-mail: asano@pu-toyama.ac.jp 2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, structure, etc.)? enzymology of peptide hydrolysis 3) What are some keywords that sum up your research involvements and interests? enzymes microorganisms enzymatic synthesis organic synthesis D-amino acid peptide ---- 1) What is your full snail-mail address, including your name and department? Synne Kleiven, Dep. of Economy, Environment and Sports, Telemark College, 3800 B=F8, Norway 2) What aspects of cyanotoxins do you work with (ie. ecological,= toxicolocial, structure, etc.)?=20 Ecological aspects 3) What are some keywords that sum up your research involvements and= interests? Environmental factors, blooming, toxinproduction, oligotrophic lakes, recreation, drinking water ------ Michael Herdman Unité de Physiologie Microbienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris cedex 15, France. Tel: (33 1) 4568 8416 Fax: (33 1) 4061 3042 mherdman@pasteur.fr Together with Rosi Rippka, I am responsible for the Pasteur Culture Collection of Cyanobacteria (PCC). Our catalogue lists about 450 strains of cyanobacteria in axenic culture, including a number of toxin-producing members of the genera Microcystis, Oscillatoria and Nodularia. Please contact me for further details of the Collection. Our work involves the general phenotypic and genotypic characterization of the strains, but we do not study toxins themselves. Keywords: DNA base composition; DNA/DNA hybridization; genotype; heterotrophy; nitrogen fixation; phenotype; phylogeny; physiology; purification; 16S rRNA. -- In response to your questions, I lead a a group who are studying a range of tropical and semi -tropical blue-green algae. Our interests are driven by the human health impacts of infestations of public water supplies in Australia. > >1) What is your full snail-mail address, including your name and > department? Professor Michael R. Moore National Research Centre for Environmental Toxicology PO box 594, Coopers Plains, Brisbane, Queensland 4108 AUSTRALIA > >2) What aspects of cyanotoxins do you work with (ie. ecological, toxicolocial, > structure, etc.)? Human toxicology, structure of toxins, function and taxonomy > >3) What are some keywords that sum up your research involvements and interests? Cylindrospermopsis, Toxins, Water supplies, Biomarkers, Interactions, Human toxicology >