Yopp Medium

NaCl116.9 g
MgSO4·7H2O10 g
MgCl2·6H2O10.68 g
KCl2 g
CaCl2·2H2O0.5 g
Ca(NO3)2·4H2O1 g
KH2PO450 mg
Ferric EDTA*5 mg
Trace metals solution1.0 ml
Deionized water to1.0 L
*acid 10% Fe
pH adjusted to 7.8 with NaOH before autoclaving.

Trace metals solution:
HCl (conc.)3.0 ml
H3BO30.5 g
MnCl2·4H2O2 g
ZnNO3·6H2O0.5 g
NaMoO4·2H2O0.025 g
CuCl2·2H2O0.025 g
Co(NO3)2·6H2O0.025 g
VOSO4·6H2O0.025 g

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