Walsby's Medium

MgSO4·7H2O0.075 g
K2HPO40.039 g
Na2CO30.02 g
CaCl2·2H2O0.018 g
H3BO32.8 mg
MnSO4·4H2O2.0 mg
ZnSO40.22 mg
MoO30.18 mg
CuSO4·5H2O0.08 mg
Co(NO3)2·6H2O0.05 mg
Iron-EDTA solution1.0 ml
Distilled water to1.0 L
pH 8.5±0.2 at room temperature

Iron-EDTA solution:
EDTA12.7 g
FeSO4·7H2O4.98 g
Distilled water to1.0 L

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