Soil/Water Biphasic (S/W) Medium


Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is seldom satisfactory.) Carefully add deionized water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilization is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0.


S/W + AMP:
S/W is prepared, but approximately 0.01 g ammonium magnesium phosphate is placed into the base of the test tube before adding the soil and water.


S/W + Ca:
S/W is prepared, but approximately 0.01 g calcium carbonate is placed into the base of the test tube before adding the soil and water.



Back to
Media Top page

Back to Cyanosite Homepage