Soil/Water Biphasic (S/W) Medium
Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam
into a test tube or jar. (The use of mud from rivers or ponds is seldom
satisfactory.) Carefully add deionized water to a depth of 7 to 10 cm, plug
or cover, and steam for one hour or autoclave for 15 minutes at 15 psi
(longer for larger vessels) on each of 2 consecutive days; further
sterilization is not needed. Allow to stand for a further day before
inoculating, when the pH should be between 7.0 - 8.0.
S/W + AMP:
S/W is prepared, but approximately 0.01 g
ammonium magnesium phosphate is placed into the base of the test tube before
adding the soil and water.
S/W + Ca:
S/W is prepared, but approximately 0.01 g
calcium carbonate is placed into the base of the test tube before adding the
soil and water.
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