Spirulina (SP) Medium


PART 1:
NaHCO327.22 g
Na2CO38.06 g
K2HPO41.00 g
Distilled water to500.0 ml

PART 2:
NaNO35.00 g
K2SO42.00 g
NaCl2.00 g
MgSO4·7H2O0.40 g
CaCl2·2H2O0.02 g
FeSO4·7H2O0.02 g
EDTANa20.16 g
Micronutrient solution10.0 ml
Vitamin solution5.00 ml
Distilled water to500.0 ml
Autoclave Parts 1 and 2 separately at 15 psi, allow to cool then mix aseptically.

Micronutrient solution:
ZnSO4·7H2O0.001 g
MnSO4·7H2O0.002 g
H3BO30.010 g
Na2MoO4·2H2O0.001 g
Co(NO3)2·6H2O0.001 g
CuSO4·5H2O0.00005 g
FeSO4·7H2O0.70 g
EDTANa20.80 g
Distilled water to1.0 L
May be stored frozen at -20 C.

Vitamin solution:
Biotin0.0002 g
Calcium pantothenate0.02 g
Cyanocobalamin0.004 g
Folic acid0.0004 g
Inositol1.00 g
Nicotinic acid0.02 g
Thiamine HCl0.10 g
Thymine0.60 g
Distilled water to1.0 L
May be stored frozen at -20 C.



Back to Media Top page

Back to Cyanosite Homepage