Modified SN Medium

Autoclave 1.0 L filtered seawater in teflon lined bottle. Add the following compounds aseptically after autoclaving:

NaNO3 (300.0 g/L dH2O)10.0 ml
K2HPO4 (anhydrous) (6.1 g/L dH2O)2.5 ml
Na2EDTA·2H2O (1.0 g/L dH2O)5.0 ml
Na2CO3 (4.0 g/L dH2O)2.5 ml
H2SeO3 (1.29 mg/L dH2O)1.0 ml
Va Vitamin Solution1.0 ml
Cyano Trace Metal Solution1.0 ml

Cyano Trace Metal Solution:
Citric Acid·H2O6.25 g
Ferric Ammonium Citrate6.0 g
MnCl2·4H2O1.4 g
Na2MoO4·2H2O0.39 g
Co(NO3)2·6H2O0.025 g
ZnSO4·7H2O0.222 g
Dissolve each metal compound individually in 100 ml dH2O. Combine and mix the six solutions, then bring volume up to 1.0 L with dH2O.

Va Vitamin Solution:
Inositol100.0 mg
Thiamine-HCl20.0 mg
Vitamin B12 (1.0 g/L dH2O)0.1 ml
Biotin (0.1 g/L dH2O)1.0 ml
Folic Acid (2.0 g/L dH2O)0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH2O)0.5 ml
Niacin (1.0 g/L dH2O)10.0 ml
Ca-Pantothenate (2.0 g/L dH2O)10.0 ml
Pyridoxine (1.0 g/L dH2O)10.0 ml
Distilled water to100 ml
Filter sterilize and freeze in plasticware.

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