NaNO3 (75.0 g/L dH2O) | 1.0 ml |
NaH2PO4·H2O (5.0 g/L dH2O) | 1.0 ml |
Alkaline Soil Extract | 15.0 ml |
f/2 Trace Metal Solution | 1.0 ml |
f/2 Vitamin Solution | 0.5 ml |
Filtered seawater to | 1.0 L |
Alkaline Soil Extract:
In a flask add two parts by volume distilled water (dH2O) to one part rich
organic garden soil (containing no recent applications of chemical
fertilizer or pesticides). Add 2-3 g NaOH/liter. Autoclave for 2 hours, and
filter when cool. This concentrated extract is diluted 1:50 in dH2O for the
final working stock. (Seawater may substituted for dH2O if the extract is to
be used for marine species).
f/2 Trace Metal Solution:
FeCl3·6H2O | 3.15 g |
Na2EDTA·2H2O | 4.36 g |
CuSO4·5H2O (9.8 g/L dH2O) | 1.0 ml |
Na2MoO4·2H2O (6.3 g/L dH2O) | 1.0 ml |
ZnSO4·7H2O (22.0 g/L dH2O) | 1.0 ml |
CoCl2·6H2O (10.0 g/L dH2O) | 1.0 ml |
MnCl2·4H2O (180.0 g/L dH2O) | 1.0 ml |
Distilled water to | 1.0 L |
f/2 Vitamin Solution:
Vitamin B12 (1.0 g/L dH2O) | 1.0 ml |
Biotin (0.1 g/L dH2O) | 10.0 ml |
Thiamine HCl | 200.0 mg |
Distilled water to | 1.0 L |
Note: Vitamin B12 and Biotin are obtained in a crystalline form. When preparing the Vitamin B12 Stock Solution allow for approximately 11% water of crystallization (For each 1.0 mg of Vitamin B12 add 0.89 ml dH2O). When preparing the Biotin Stock Solution allow for approximately 4% water of crystallization (For each 1.0 mg of Biotin add 9.6 ml dH2O).
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