| NaH2PO4·H2O solution (6.9 g/L) | 1 ml |
| NH4Cl solution (42.8 g/L) | 1 ml |
| Trace element solution | 1 ml |
| Oligotrophic seawater | to 1 L |
Acid wash and dH2O rinse all glassware. Use oligotrophic (Sargasso Sea) seawater. Autoclave and cool before adding filter-sterilized nutrient stock solutions.
Trace element solution:
| Na2EDTA·2 H2O | 0.436 g |
| FeCl3·6 H2O | 0.316 g |
| ZnSO4·7 H2O solution (2.3 g/L) | 1 ml |
| CoCl2·6 H2O solution (1.19 g/L) | 1 ml |
| MnCl2·4 H2O solution (17.8 g/L) | 1 ml |
| Na2MoO4·2 H2O solution (0.73 g/L) | 1 ml |
| Na2SeO3 solution (1.29 g/L) | 1 ml |
| NiSO4·6 H2O solution (2.63 g/L) | 1 ml |
| Distilled water | to 1 L |
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