CRBIP Medium 1559
Prochlorococcus Medium


Turks Island Salts 1X250 ml
Iron EDTA solution1 ml
Trace metals0.25 ml
Sodium phosphate solution0.25 ml
Ammonium sulfate solution1 ml
Sodium hydrogenocarbonate solution0.5 ml
Vitamin B12 solution0.25 ml

Turks Island Salts 1X:
NaCl28 g
KCl0.67 g
MgCl2·6 H2O5.5 g
MgSO4·7 H2O6.9 g
CaCl2·2 H2O1.45 g
Distilled H2Oto 1 L

Sodium hydrogenocarbonate solution:
NaHCO325.2 g
Distilled waterto 300 ml

Iron EDTA solution:
FeCl3·6 H2O0.27 g
Na3EDTA·2 H2O0.372 g
Distilled waterto 500 ml

Sodium Phosphate solution:
Solution A: 3.45 g NaH2PO4·2 H2O in 50 ml H2O
Solution B: 4.45 g NaHPO4·2 H2O in 50 ml H2O
Use solution B to adjust solution A to pH 7.5

Ammonium sulfate solution:
(NH4)2SO43.3 g
Distilled waterto 250 ml

Vitamin B12 solution:
Vitamin B1220 mg
Distilled waterto 20 ml

Filter sterilize.

Trace metals:
H3BO32.86 g
MnCl2·4 H2O1.81 g
ZnSO4·7 H2O0.222 g
Na2MoO4·2 H2O0.39 g
Co(NO3)2·6 H2O0.049 g
Distilled waterto 1 L



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