| ATCC 616 Medium: BG11 Medium | 75 ml |
| Turks Island Salts 4X (1:1) | 25 ml |
| Sodium hydrogenocarbonate solution | 0.5 ml |
Turks Island Salts 4X:
| NaCl | 112 g |
| KCl | 2.68 g |
| MgCl2·6 H2O | 22 g |
| MgSO4·7 H2O | 27.7 g |
| CaCl2·2 H2O | 5.8 g |
| Distilled H2O | to 1 L |
Sodium hydrogenocarbonate solution:
| NaHCO3 | 25.2 g |
| Distilled water | to 300 ml |
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