| Macro component 1 | 10 ml |
| Macro component 2 | 10 ml |
| CaCl2·2H2O solution (2.9 g/L) | 1 ml |
| G9 trace metals | 1 ml |
| Deionized water to | 1 L |
Final pH 8.3-8.5
Macro component 1:
| NaNO3 | 67 g |
| Na2HPO4 | 0.67 g |
| K2HPO4 | 3.8 g |
| Tricine | 25 g |
| Deionized water to | 1 L |
pH 8.5-8.8
Macro component 2:
| Na2EDTA·2H2O | 4.4 g |
| MgSO4·7H2O | 10 g |
| MgCl2·6H2O | 5 g |
| FeCl3·6H2O | 0.5 g |
| Deionized water to | 1 L |
pH 7.5
G9 trace metals:
| H3BO3 | 3.25 g |
| MnSO4·H2O | 1.5 g |
| ZnSO4·7H2O | 0.3 g |
| (NH4)6Mo7O24·4H2O | 0.08 g |
| CuSO4·5H2O | 0.05 g |
| Co(NO3)2·6H2O | 0.15 g |
| KI | 0.01 g |
| Deionized water to | 1 L |
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