J Medium

Modification of Parker's J (or, Jansen) medium for Microcystis

Macro component 110 ml
Macro component 210 ml
CaCl2·2H2O solution (2.9 g/L)1 ml
G9 trace metals1 ml
Deionized water to1 L

Final pH 8.3-8.5

Macro component 1:
NaNO367 g
Na2HPO40.67 g
K2HPO43.8 g
Tricine25 g
Deionized water to1 L

pH 8.5-8.8

Macro component 2:
Na2EDTA·2H2O4.4 g
MgSO4·7H2O10 g
MgCl2·6H2O5 g
FeCl3·6H2O0.5 g
Deionized water to1 L

pH 7.5

G9 trace metals:
H3BO33.25 g
MnSO4·H2O1.5 g
ZnSO4·7H2O0.3 g
(NH4)6Mo7O24·4H2O0.08 g
CuSO4·5H2O0.05 g
Co(NO3)2·6H2O0.15 g
KI0.01 g
Deionized water to1 L

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