PART 1:
| Tricine | 0.50 g |
| Soil extract (SE1) | 25.00 ml |
| Extra nutrient salts | 3.75 ml |
| Filtered natural seawater to | 1.0 L |
PART 2:
| NaNO3 | 1.500 g |
| K2HPO4·3H2O | 0.040 g |
| MgSO4·7H2O | 0.075 g |
| CaCl2·2H2O | 0.036 g |
| Citric acid | 0.006 g |
| Ammonium ferric citrate green | 0.006 g |
| EDTANa2 | 0.001 g |
| Na2CO3 | 0.020 g |
| Trace metal solution | 1.00 ml |
| Distilled water to | 1.0 L |
Extra nutrient salts:
| NaNO3 | 3.00 g |
| Na2HPO4 | 0.12 g |
| K2HPO4 | 0.10 g |
| Distilled water to | 100.0 ml |
Trace metal solution:
| H3BO3 | 0.286 g |
| MnCl2·4H2O | 0.022 g |
| Na2MoO4·2H2O | 0.039 g |
| CuSO4·5H2O | 0.008 g |
| Co(NO3)2·6H2O | 0.005 g |
| Distilled water to | 100.0 ml |
Soil Extract (SE1):
Site selection for a good soil is very important and for most purposes a
soil from undisturbed deciduous woodland is best. Sites to avoid are those
showing obvious signs of man's activity and particular care should be taken
to avoid areas where fertilizers, crop sprays or other toxic chemicals may
have been used.
A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2-4 mm) or stored as it is prior to use.
Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No. 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (eg., 1 L or less for 15 minutes) and are then kept in a cool place (eg., a refrigerator) until required.
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