Bold's Basal (BB) Medium

NaNO3 (5.0 g/200 ml)10.0 ml
MgSO4·7H2O (1.5 g/200 ml)10.0 ml
NaCl (0.5 g/200 ml)10.0 ml
K2HPO4 (1.5 g/200 ml)10.0 ml
KH2PO4 (3.5 g/200 ml)10.0 ml
CaCl2·2H2O (0.5 g/200 ml)10.0 ml
H3BO3 (1.14 g/100 ml)1.0 ml
Trace elements solution1.0 ml
EDTA stock1.0 ml
Fe solution1.0 ml
Distilled water to1.0 L
For agar, add 15.0 g/L Bacteriological Agar* (Oxoid L11). Autoclave at 15 psi for 15 minutes.

*Supplier: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK

Trace elements solution:
ZnSO4·7H2O8.82 g
MnCl2·4H2O1.44 g
MoO30.71 g
CuSO4·5H2O1.57 g
Co(NO3)2·6H2O0.49 g
Distilled water to1.0 L
May need autoclaving to dissolve.

EDTA stock:
EDTANa25.0 g
KOH3.1 g
Distilled water to100 ml

Fe solution:
FeSO4·7H2O4.98 g
conc H2SO41.0 ml
Distilled water to1.0 L

Back to Media Top page

Back to Cyanosite Homepage