Z8 Medium

MgSO4·7H2O0.25 g
NaNO30.467 g
Ca(NO3)2·4H2O59 mg
NH4Cl31 mg
Na2CO30.02 g
FeEDTA solution10 ml
Gaffron micronutrients1.0 ml
Deionized water to1.0 L

FeEDTA solution:
Made in two solutions:
Solution A - 2.8 g FeCl3 in 100 ml 0.1 N HCl
Solution B - 3.9 g EDTANa2 in 100 ml 0.1 N NaOH
Add 10 ml solution A and 9.5 ml solution B plus water to 1 L.

Gaffron micronutrients:
H3BO33.1 g
MnSO4·4H2O2.23 g
ZnSO4·7H2O0.22 g
(NH4)6Mo7O24·4H2O0.088 g
Co(NO3)2·6H2O0.146 g
VOSO4·6H2O0.054 g
Al2(SO4)3K2SO4·2H2O0.474 g
NiSO4(NH4)2SO4·6H2O0.198 g
Cd(NO3)2·4H2O0.154 g
Cr(NO3)3·7H2O0.037 g
Na2WO4·2H2O0.033 g
KBr0.119 g
KI0.083 g
Deionized water to1 L

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