SOT Medium for Spirulina

ATCC Medium 1679

NaHCO316.8 g
K2HPO40.5 g
NaNO32.5 g
K2SO41.0 g
NaCl1.0 g
MgSO4·7H2O0.2 g
CaCl2·2H2O0.04 g
FeSO4·7H2O0.01 g
Na2EDTA·2H2O0.08 g
Trace mineral mix A51.0 ml
Trace mineral mix B6 Modified1.0 ml
Distilled water1.0 L
Adjust medium for final pH 9.0.

Trace mineral mix B6 Modified:
NH4NO30.23 g
K2Cr2(SO4)4·24H2O96.0 mg
NiSO4·7H2O47.8 mg
Na2WO4·2H2O17.9 mg
Ti2(SO4)340.0 mg
Distilled water1.0 L

Trace metal mix A5:
H3BO32.86 g
MnCl2·4H2O1.81 g
ZnSO4·7H2O0.222 g
NaMoO4·2H2O0.39 g
CuSO4·5H2O0.079 g
Co(NO3)2·6H2O49.4 mg
Distilled water1.0 L

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