SN Medium

Mix 750 ml filtered seawater and 250 ml distilled H2O. Autoclave in a teflon lined bottle then add the following compounds aseptically:

NaNO3 (300.0 g/L dH2O)2.5 ml
K2HPO4 (anhydrous) (6.1 g/L dH2O)2.6 ml
Na2EDTA·2H2O (1.0 g/L dH2O)5.6 ml
Na2CO3 (4.0 g/L dH2O)2.6 ml
Vitamin B12 (1.0 mg/L dH2O)1.0 ml
Cyano Trace Metal Solution1.0 ml

Cyano Trace Metal Solution:
Citric Acid·H2O6.25 g
Ferric Ammonium Citrate6.0 g
MnCl2·4H2O1.4 g
Na2MoO4·2H2O0.39 g
Co(NO3)2·6H2O0.025 g
ZnSO4·7H2O0.222 g
Dissolve each metal compound individually in 100 ml dH2O. Combine and mix the six solutions, then bring volume up to 1.0 L with dH2O.

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