MN Marine Medium

ATCC Medium 957

Glass-distilled water250.0 ml
Seawater750.0 ml
MgSO4·7H2O0.04 g
CaCl2·2H2O0.02 g
NaNO30.75 g
K2HPO4·3H2O0.02 g
Citric acid3.0 mg
Ferric ammonium citrate3.0 mg
EDTA (disodium potassium salt)0.5 mg
NaCO30.02 g
Trace metals A-51.0 ml
Noble agar (if needed)10.0 g
Adjust pH after autoclaving to 8.5 with KOH

Trace metal mix A5:
H3BO32.86 g
MnCl2·4H2O1.81 g
ZnSO4·7H2O0.222 g
NaMoO4·2H2O0.39 g
CuSO4·5H2O0.079 g
Co(NO3)2·6H2O49.4 mg
Distilled water1.0 L

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