L1 Medium and Derivatives

NaNO3 (75.0 g/L dH2O)1.0 ml
NaH2PO4·H2O (5.0 g/L dH2O)1.0 ml
Na2SiO3·9H2O (30.0 g/L dH2O)1.0 ml
L1 Trace Metal Solution1.0 ml
f/2 Vitamin Solution0.5 ml
Filtered seawater to1.0 L
Autoclave after all additions.

L1 Trace Metal Solution:
FeCl3·6H2O3.15 g
Na2EDTA·2H2O4.36 g
CuSO4·5H2O (9.8 g/L dH2O)0.25 ml
Na2MoO4·2H2O (6.3 g/L dH2O)3.0 ml
ZnSO4·7H2O (22.0 g/L dH2O)1.0 ml
CoCl2·6H2O (10.0 g/L dH2O)1.0 ml
MnCl2·4H2O (180.0 g/L dH2O)1.0 ml
H2SeO3 (1.3 g/L dH2O)1.0 ml
NiSO4·6H2O (2.7 g/L dH2O)1.0 ml
Na3VO4 (1.84 g/L dH2O)1.0 ml
K2CrO4 (1.94 g/L dH2O)1.0 ml
Filtered seawater to1.0 L

f/2 Vitamin Solution:
Vitamin B12 (1.0 g/L dH2O)1.0 ml
Biotin (0.1 g/L dH2O)10.0 ml
Thiamine HCl200.0 mg
Distilled water to1.0 L
Filter sterilize into plastic vials and store in refrigerator.

Note: Vitamin B12 and Biotin are obtained in a crystalline form. When preparing the Vitamin B12 Stock Solution allow for approximately 11% water of crystallization (For each 1.0 mg of Vitamin B12 add 0.89 ml dH2O). When preparing the Biotin Stock Solution allow for approximately 4% water of crystallization (For each 1.0 mg of Biotin add 9.6 ml dH2O).

L1 Derivatives

L1-Si:
Prepare as for L1 Medium but omit Na2SiO3·9H2O.

L1-Si (80%SW):
This medium is used to maintain organisms from brackish waters (approximately 26 ppt salinity). Mix 800 ml filtered seawater to 200 ml dH2O. Prepare L1-Si Medium in the diluted seawater, then autoclave.

L2 Trace Metal Solution:
This trace metal solution is similar to L1 Trace Metal Solution, but contains raised levels of Na2EDTA·2H2O. The higher chelation is favoured by some oceanic strains. Prepare using the recipe for L1 Trace Metal Solution, but add a total of 37.2 g of Na2EDTA·2H2O.


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