NaNO3 (75.0 g/L dH2O) | 1.0 ml |
NH4Cl (2.68 g/L dH2O) | 1.0 ml |
beta-Glycerophosphate (2.16 g/L dH2O) | 1.0 ml |
Na2SiO3·9H2O (30.0 g/L dH2O) | 1.0 ml |
H2SeO3 (1.29 mg/L dH2O) | 1.0 ml |
TRIS-Base [pH 7.2] (121.1 g/L dH2O) | 1.0 ml |
K Trace Metal Solution | 1.0 ml |
f/2 Vitamin Solution | 0.5 ml |
Filtered seawater to | 1.0 L |
K Trace Metal Solution:
Na2EDTA·2H2O | 41.6 g |
FeCl3·6H2O | 3.15 g |
Na2MoO4·2H2O (6.3 g/L dH2O) | 1.0 ml |
ZnSO4·7H2O (22.0 g/L dH2O) | 1.0 ml |
CoCl2·6H2O (10.0 g/L dH2O) | 1.0 ml |
MnCl2·4H2O (180.0 g/L dH2O) | 1.0 ml |
CuSO4·5H2O (9.8 g/L dH2O) | 0.5 ml |
Distilled water to | 1.0 L |
f/2 Vitamin Solution:
Vitamin B12 (1.0 g/L dH2O) | 1.0 ml |
Biotin (0.1 g/L dH2O) | 10.0 ml |
Thiamine HCl | 200.0 mg |
Distilled water to | 1.0 L |
Note: Vitamin B12 and Biotin are obtained in a crystalline form. When preparing the Vitamin B12 Stock Solution allow for approximately 11% water of crystallization (For each 1.0 mg of Vitamin B12 add 0.89 ml dH2O). When preparing the Biotin Stock Solution allow for approximately 4% water of crystallization (For each 1.0 mg of Biotin add 9.6 ml dH2O).
Back to Cyanosite Homepage