K Medium

NaNO3 (75.0 g/L dH2O)1.0 ml
NH4Cl (2.68 g/L dH2O)1.0 ml
beta-Glycerophosphate (2.16 g/L dH2O)1.0 ml
Na2SiO3·9H2O (30.0 g/L dH2O)1.0 ml
H2SeO3 (1.29 mg/L dH2O)1.0 ml
TRIS-Base [pH 7.2] (121.1 g/L dH2O)1.0 ml
K Trace Metal Solution1.0 ml
f/2 Vitamin Solution0.5 ml
Filtered seawater to1.0 L
Autoclave after all additions.

K Trace Metal Solution:
Na2EDTA·2H2O41.6 g
FeCl3·6H2O3.15 g
Na2MoO4·2H2O (6.3 g/L dH2O)1.0 ml
ZnSO4·7H2O (22.0 g/L dH2O)1.0 ml
CoCl2·6H2O (10.0 g/L dH2O)1.0 ml
MnCl2·4H2O (180.0 g/L dH2O)1.0 ml
CuSO4·5H2O (9.8 g/L dH2O)0.5 ml
Distilled water to1.0 L
Heat to dissolve.

f/2 Vitamin Solution:
Vitamin B12 (1.0 g/L dH2O)1.0 ml
Biotin (0.1 g/L dH2O)10.0 ml
Thiamine HCl200.0 mg
Distilled water to1.0 L
Filter sterilize into plastic vials and store in refrigerator.

Note: Vitamin B12 and Biotin are obtained in a crystalline form. When preparing the Vitamin B12 Stock Solution allow for approximately 11% water of crystallization (For each 1.0 mg of Vitamin B12 add 0.89 ml dH2O). When preparing the Biotin Stock Solution allow for approximately 4% water of crystallization (For each 1.0 mg of Biotin add 9.6 ml dH2O).

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