| NaCl | 160.0 g |
| NaNO3 | 0.69 g |
| Na2HPO4 | 0.111 g |
| KNO3 | 0.103 g |
| MgSO4·7H2O | 0.1 g |
| Nitrilotriacetic acid | 0.1 g |
| CaSO4·2H2O | 0.06 g |
| FeCl3 | 0.3 mg |
| Castenholtz trace metal solution | 1.0 ml |
Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.
Castenholtz trace metal solution:
| MnSO4·H2O | 2.28 g |
| H3BO3 | 0.5 g |
| ZnSO4·7H2O | 0.5 g |
| Co(NO3)2·6H2O | 0.025 g |
| CuSO4·5H2O | 0.025 g |
| Na2MoO4·2H2O | 0.025 g |
| H2SO4 | 0.5 ml |
| Distilled water to | 1.0 L |
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