DCM Medium

TRIS-Base5.0 g
Sodium Acetate·3H2O0.5 g
Monosodium Glutamate0.5 g
D,L-Glycine0.5 g
Sucrose0.5 g
beta-Glycerophosphate0.4 g
NaNO3 (75.0 g/L dH2O)6.7 ml
Glycerine (100.0 g/L dH2O)5.0 ml
NH4Cl (2.68 g/L dH2O)1.0 ml
H2SeO3 (1.29 mg/L dH2O)1.0 ml
Lactic Acid1.0 ml
DCM Trace Metal Solution1.0 ml
Va Vitamin Solution1.0 ml
Filtered natural seawater to1.0 L
Solid compounds should be dissolved in dH2O prior to addition. Adjust pH to 7.6-8.0 (with 3N HCl). Autoclave.

DCM Trace Metal Solution:
Na2EDTA·2H2O29.36 g
FeCl3·6H2O3.15 g
ZnSO4·7H2O (22.0 g/L dH2O)30.0 ml
MnCl2·4H2O (180.0 g/L dH2O)20.0 ml
Na2MoO4·2H2O (6.3 g/L dH2O)1.0 ml
CoCl2·6H2O (10.0 g/L dH2O)1.0 ml
CuSO4·5H2O (9.8 g/L dH2O)0.5 ml
Distilled water to1.0 L

Va Vitamin Solution:
Inositol100.0 mg
Thiamine-HCl20.0 mg
Vitamin B12 (1.0 g/L dH2O)0.1 ml
Biotin (0.1 g/L dH2O)1.0 ml
Folic Acid (2.0 g/L dH2O)0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH2O)0.5 ml
Niacin (1.0 g/L dH2O)10.0 ml
Ca-Pantothenate (2.0 g/L dH2O)10.0 ml
Pyridoxine (1.0 g/L dH2O)10.0 ml
Distilled water to100 ml
Filter sterilize and freeze in plasticware.

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