Ashbey's Nitrogen-free Agar
Agar
15.0 g
Mannitol
15.0 g
CaCl
2
·2H
2
O
0.2 g
K
2
HPO
4
0.2 g
MgSO
4
·7H
2
O
0.2 g
MoO
3
(10% solution)
0.1 ml
FeCl
3
(10% solution)
0.05 ml
Distilled water to
1.0 L
pH 7.2±0.2 at room temperature
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