ASP 2 Medium

Na₂EDTA (3.0 g/L)	10 ml
P1 metals	1.0 ml
Iron solution	1.0 ml
KCl (60 g/L)	10 ml
CaCl₂·2H₂O (37 g/L)	10 ml
K₂HPO₄ (50 g/L)	1.0 ml
NaCl	18 g
Buffer solution	10 ml
MgSO₄	2.44 g
Deionized water to	1.0 L

Iron solution:

FeCl₃·6H₂O 1.95 g
10 M HCl 5 ml
Deionized water to 500 ml
Filter sterilize.

Buffer solution:

TAPS 100 g
TAPSO 100 g
Deionized water to 1.0 L
pH 7.7

P1 metals:

H₃BO₃ 17.15 g
MnCl₂·4H₂O 2.16 g
ZnSO₄·7H₂O 0.334 g
CuSO₄·5H₂O (0.1 g/5 ml)* 0.075 ml
CoCl₂·6H₂O (0.1 g/5 ml)* 0.3 ml
Na₂MoO₄·2H₂O (0.11 g/5 ml)* 1.0 ml
Deionized water to 500 ml
*make these stocks fresh and discard after use.

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H₃BO₃	17.15 g
MnCl₂·4H₂O	2.16 g
ZnSO₄·7H₂O	0.334 g
CuSO₄·5H₂O (0.1 g/5 ml)*	0.075 ml
CoCl₂·6H₂O (0.1 g/5 ml)*	0.3 ml
Na₂MoO₄·2H₂O (0.11 g/5 ml)*	1.0 ml
Deionized water to	500 ml