Walsby's Medium
MgSO4 7H2O 0.075 g
K2HPO4 0.039 g
Na2CO3 0.02 g
CaCl2 2H2O 0.018 g
H3BO3 2.8 mg
MnSO4 4H2O 2.0 mg
ZnSO4 0.22 mg
MoO3 0.18 mg
CuSO4 5H2O 0.08 mg
Co(NO3)2 6H2O 0.05 mg
Iron-EDTA solution 1.0 ml
Distilled water to 1.0 L
pH 8.5 +/- 0.2 at room temperature
Iron-EDTA solution:
EDTA 12.7 g
FeSO4 7H2O 4.98 g
Distilled water to 1.0 L
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