PART 2:
NaNO₃ 5.00 g
K₂SO₄ 2.00 g
NaCl 2.00 g
MgSO₄ 7H₂O 0.40 g
CaCl₂ 2H₂O 0.02 g
FeSO₄ 7H₂O 0.02 g
EDTANa₂ 0.16 g
Micronutrient solution 10.0 ml
Vitamin solution 5.00 ml
Distilled water to 500.0 ml

Autoclave Parts 1 and 2 separately at 15 psi, allow to cool then mix aseptically.

Micronutrient solution:
ZnSO₄ 7H₂O 0.001 g
MnSO₄ 7H₂O 0.002 g
H₃BO₃ 0.010 g
Na₂MoO₄ 2H₂O 0.001 g
Co(NO₃)₂ 6H₂O 0.001 g
CuSO₄ 5H₂O 0.00005 g
FeSO₄ 7H₂O 0.70 g
EDTANa₂ 0.80 g
Distilled water to 1.0 L

May be stored frozen at -20 C.

Vitamin solution:
Biotin 0.0002 g
Calcium pantothenate 0.02 g
Cyanocobalamin 0.004 g
Folic acid 0.0004 g
Inositol 1.00 g
Nicotinic acid 0.02 g
Thiamine HCl 0.10 g
Thymine 0.60 g
Distilled water to 1.0 L

May be stored frozen at -20 C.

Back to Cyanosite Homepage