Acid wash and dH2O rinse all glassware. Use oligotrophic (Sargasso Sea) seawater. Autoclave and cool before adding filter-sterilized nutrient stock solutions.
Trace element solution:
Na2EDTA 2 H2O 0.436 g
FeCl3 6 H2O 0.316 g
ZnSO4 7 H2O solution (2.3 g/L) 1 ml
CoCl2 6 H2O solution (1.19 g/L) 1 ml
MnCl2 4 H2O solution (17.8 g/L) 1 ml
Na2MoO4 2 H2O solution (0.73 g/L) 1 ml
Na2SeO3 solution (1.29 g/L) 1 ml
NiSO4 6 H2O solution (2.63 g/L) 1 ml
Distilled water to 1 L
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