Pro99 Medium
Prochlorococcus Medium



NaH2PO4 H2O solution (6.9 g/L) 1 ml
NH4Cl solution (42.8 g/L) 1 ml
Trace element solution 1 ml
Oligotrophic seawater to 1 L

Acid wash and dH2O rinse all glassware. Use oligotrophic (Sargasso Sea) seawater. Autoclave and cool before adding filter-sterilized nutrient stock solutions.

Trace element solution:

Na2EDTA 2 H2O 0.436 g FeCl3 6 H2O 0.316 g
ZnSO4 7 H2O solution (2.3 g/L) 1 ml
CoCl2 6 H2O solution (1.19 g/L) 1 ml
MnCl2 4 H2O solution (17.8 g/L) 1 ml
Na2MoO4 2 H2O solution (0.73 g/L) 1 ml
Na2SeO3 solution (1.29 g/L) 1 ml
NiSO4 6 H2O solution (2.63 g/L) 1 ml
Distilled water to 1 L



Back to Media Top page

Back to Cyanosite Homepage