CRBIP Medium 1559
Prochlorococcus Medium



Turks Island Salts 1X 250 ml
Iron EDTA solution 1 ml
Trace metals 0.25 ml
Sodium phosphate solution 0.25 ml
Ammonium sulfate solution 1 ml
Sodium hydrogenocarbonate solution 0.5 ml
Vitamin B12 solution 0.25 ml

Turks Island Salts 1X:

NaCl 28 g
KCl 0.67 g
MgCl2 6 H2O 5.5 g
MgSO4 7 H2O 6.9 g
CaCl2 2 H2O 1.45 g
Distilled H2O to 1 L

Sodium hydrogenocarbonate solution:

NaHCO3 25.2 g
Distilled water to 300 ml

Iron EDTA solution:

FeCl3 6 H2O 0.27 g
Na3EDTA 2 H2O 0.372 g
Distilled water to 500 ml

Sodium Phosphate solution:
Solution A: 3.45 g NaH2PO4 2 H2O in 50 ml H2O
Solution B: 4.45 g NaHPO4 2 H2O in 50 ml H2O
Use solution B to adjust solution A to pH 7.5

Ammonium sulfate solution:

(NH4)2SO4 3.3 g
Distilled water to 250 ml

Vitamin B12 solution:

Vitamin B12 20 mg
Distilled water to 20 ml

Filter sterilize.

Trace metals:

H3BO3 2.86 g
MnCl2 4 H2O 1.81 g
ZnSO4 7 H2O 0.222 g
Na2MoO4 2 H2O 0.39 g
Co(NO3)2 6 H2O 0.049 g
Distilled water to 1 L



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