Macro component 1 10 ml
Macro component 2 10 ml
CaCl₂ 2H₂O solution (2.9 g/L) 1 ml
G9 trace metals 1 ml
Deionized water to 1 L

Final pH 8.3-8.5

Macro component 1:

NaNO₃ 67 g
Na₂HPO₄ 0.67 g
K₂HPO₄ 3.8 g
Tricine 25 g
Deionized water to 1 L

pH 8.5-8.8

Macro component 2:

Na₂EDTA 2H₂O 4.4 g
MgSO₄ 7H₂O 10 g
MgCl₂ 6H₂O 5 g
FeCl₃ 6H₂O 0.5 g
Deionized water to 1 L

pH 7.5

G9 trace metals:

H₃BO₃ 3.25 g
MnSO₄ H₂O 1.5 g
ZnSO₄ 7H₂O 0.3 g
(NH₄)₆Mo₇O₂₄ 4H₂O 0.08 g
CuSO₄ 5H₂O 0.05 g
Co(NO₃)₂ 6H₂O 0.15 g
KI 0.01 g
Deionized water to 1 L

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