Autoclave after all additions.

Note: f/2 Medium contains extensive silica precipitate and should be used only when growing diatoms. For other algal groups use f/2-Si Medium.

f/2 Trace Metal Solution:
FeCl₃ 6H₂O 3.15 g
Na₂EDTA 2H₂O 4.36 g
CuSO₄ 5H₂O (9.8 g/L dH₂O) 1.0 ml
Na₂MoO₄ 2H₂O (6.3 g/L dH₂O) 1.0 ml
ZnSO₄ 7H₂O (22.0 g/L dH₂O) 1.0 ml
CoCl₂ 6H₂O (10.0 g/L dH₂O) 1.0 ml
MnCl₂ 4H₂O (180.0 g/L dH₂O) 1.0 ml
Distilled water to 1.0 L

Autoclave.

f/2 Vitamin Solution:
Vitamin B₁₂ (1.0 g/L dH₂O) 1.0 ml
Biotin (0.1 g/L dH₂O) 10.0 ml
Thiamine HCl 200.0 mg
Distilled water to 1.0 L
Filter sterilize into plastic vials and store in refrigerator.

Note: Vitamin B₁₂ and Biotin are obtained in a crystalline form. When preparing the Vitamin B₁₂ Stock Solution allow for approximately 11% water of crystallization (For each 1.0 mg of Vitamin B₁₂ add 0.89 ml dH₂O). When preparing the Biotin Stock Solution allow for approximately 4% water of crystallization (For each 1.0 mg of Biotin add 9.6 ml dH₂O).

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