Bold's Basal (BB) Medium

NaNO3 (5.0 g/200 ml) 10.0 ml
MgSO4 7H2O (1.5 g/200 ml) 10.0 ml
NaCl (0.5 g/200 ml) 10.0 ml
K2HPO4 (1.5 g/200 ml) 10.0 ml
KH2PO4 (3.5 g/200 ml) 10.0 ml
CaCl2 2H2O (0.5 g/200 ml) 10.0 ml
H3BO3 (1.14 g/100 ml) 1.0 ml
Trace elements solution 1.0 ml
EDTA stock 1.0 ml
Fe solution 1.0 ml
Distilled water to 1.0 L

For agar, add 15.0 g/L Bacteriological Agar* (Oxoid L11). Autoclave at 15 psi for 15 minutes.

*Supplier: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK

Trace elements solution:
ZnSO4 7H2O 8.82 g
MnCl2 4H2O 1.44 g
MoO3 0.71 g
CuSO4 5H2O 1.57 g
Co(NO3)2 6H2O 0.49 g
Distilled water to 1.0 L

May need autoclaving to dissolve.

EDTA stock:
EDTANa2 5.0 g
KOH 3.1 g
Distilled water to 100 ml

Fe solution:
FeSO4 7H2O 4.98 g
conc H2SO4 1.0 ml
Distilled water to 1.0 L


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