Filter sterilize. Do not autoclave.
Trace metal solution:
Na2EDTA 2 H2O 0.745 g
FeCl3 6 H2O solution (0.11 g/L) 1 ml
MnCl2 4 H2O solution (3.96 g/L) 1 ml
ZnSO4 7 H2O solution (1.15 g/L) 1 ml
Na2MoO4 2 H2O solution (2.66 g/L) 1 ml
CuSO4 5 H2O solution (2.23 g/L) 1 ml
CoCl2 6 H2O solution (5.95 g/L) 1 ml
Distilled water to 1 L
Vitamin solution:
Thiamine HCl 100 mg
Biotin solution (0.5 g/L) 1 ml
Cyanocobalamin solution (0.5 g/L) 1 ml
Distilled water to 1 L
Chen, Y.-B., Zehr, J.P. & Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp. IMS 101 in defined media: evidence for a circadian rhythm. J. Phycol. 32: 916-923.
Back to Cyanosite Homepage