SOT Medium for Spirulina

ATCC Medium 1679

NaHCO3 16.8 g
K2HPO4 0.5 g
NaNO3 2.5 g
K2SO4 1.0 g
NaCl 1.0 g
MgSO4 7H2O 0.2 g
CaCl2 2H2O 0.04 g
FeSO4 7H2O 0.01 g
Na2EDTA 2H2O 0.08 g
Trace mineral mix A5 1.0 ml
Trace mineral mix B6 Modified 1.0 ml
Distilled water 1.0 L

Adjust medium for final pH 9.0.

Trace mineral mix B6 Modified:
NH4NO3 0.23 g
K2Cr2(SO4)4 24H2O 96.0 mg
NiSO4 7H2O 47.8 mg
Na2WO4 2H2O 17.9 mg
Ti2(SO4)3 40.0 mg
Distilled water 1.0 L

Trace metal mix A5:
H3BO3 2.86 g
MnCl2 4H2O 1.81 g
ZnSO4 7H2O 0.222 g
NaMoO4 2H2O 0.39 g
CuSO4 5H2O 0.079 g
Co(NO3)2 6H2O 49.4 mg
Distilled water 1.0 L


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