MN Marine Medium

ATCC Medium 957

Glass-distilled water 250.0 ml
Seawater 750.0 ml
MgSO4 7H2O 0.04 g
CaCl2 2H2O 0.02 g
NaNO3 0.75 g
K2HPO4 3H2O 0.02 g
Citric acid 3.0 mg
Ferric ammonium citrate 3.0 mg
EDTA (disodium potassium salt) 0.5 mg
NaCO3 0.02 g
Trace metals A-5 1.0 ml
Noble agar (if needed) 10.0 g

Adjust pH after autoclaving to 8.5 with KOH

Trace metal mix A5:
H3BO3 2.86 g
MnCl2 4H2O 1.81 g
ZnSO4 7H2O 0.222 g
NaMoO4 2H2O 0.39 g
CuSO4 5H2O 0.079 g
Co(NO3)2 6H2O 49.4 mg
Distilled water 1.0 L

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