Castenholtz D Medium Modified

Halophilic cyanobacteria

NaCl 160.0 g
NaNO3 0.69 g
Na2HPO4 0.111 g
KNO3 0.103 g
MgSO4 7H2O 0.1 g
Nitrilotriacetic acid 0.1 g
CaSO4 2H2O 0.06 g
FeCl3 0.3 mg
Castenholtz trace metal solution 1.0 ml

pH 7.5 +/- 0.2 at room temperature

Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.

Castenholtz trace metal solution:
MnSO4 H2O 2.28 g
H3BO3 0.5 g
ZnSO4 7H2O 0.5 g
Co(NO3)2 6H2O 0.025 g
CuSO4 5H2O 0.025 g
Na2MoO4 2H2O 0.025 g
H2SO4 0.5 ml
Distilled water to 1.0 L

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