Solid compounds should be dissolved in dH₂O prior to addition. Adjust pH to 7.6-8.0 (with 3N HCl). Autoclave.

DCM Trace Metal Solution:
Na₂EDTA 2H₂O 29.36 g
FeCl₃ 6H₂O 3.15 g
ZnSO₄ 7H₂O (22.0 g/L dH₂O) 30.0 ml
MnCl₂ 4H₂O (180.0 g/L dH₂O) 20.0 ml
Na₂MoO₄ 2H₂O (6.3 g/L dH₂O) 1.0 ml
CoCl₂ 6H₂O (10.0 g/L dH₂O)1.0 ml
CuSO₄ 5H₂O (9.8 g/L dH₂O) 0.5 ml
Distilled water to 1.0 L

Va Vitamin Solution:
Inositol 100.0 mg
Thiamine-HCl 20.0 mg
Vitamin B₁₂ (1.0 g/L dH₂O) 0.1 ml
Biotin (0.1 g/L dH₂O) 1.0 ml
Folic Acid (2.0 g/L dH₂O) 0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH₂O) 0.5 ml
Niacin (1.0 g/L dH₂O)10.0 ml
Ca-Pantothenate (2.0 g/L dH₂O) 10.0 ml
Pyridoxine (1.0 g/L dH₂O) 10.0 ml
Distilled water to 100 ml

Filter sterilize and freeze in plasticware.

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