Solid compounds should be dissolved in dH2O prior to addition. Adjust pH to 7.6-8.0 (with 3N HCl). Autoclave.
DCM Trace Metal Solution:
Na2EDTA 2H2O 29.36 g
FeCl3 6H2O 3.15 g
ZnSO4 7H2O (22.0 g/L dH2O) 30.0 ml
MnCl2 4H2O (180.0 g/L dH2O) 20.0 ml
Na2MoO4 2H2O (6.3 g/L dH2O) 1.0 ml
CoCl2 6H2O (10.0 g/L dH2O)1.0 ml
CuSO4 5H2O (9.8 g/L dH2O) 0.5 ml
Distilled water to 1.0 L
Va Vitamin Solution:
Inositol 100.0 mg
Thiamine-HCl 20.0 mg
Vitamin B12 (1.0 g/L dH2O) 0.1 ml
Biotin (0.1 g/L dH2O) 1.0 ml
Folic Acid (2.0 g/L dH2O) 0.1 ml
p-Aminobenzoic Acid (2.0 g/L dH2O) 0.5 ml
Niacin (1.0 g/L dH2O)10.0 ml
Ca-Pantothenate (2.0 g/L dH2O) 10.0 ml
Pyridoxine (1.0 g/L dH2O) 10.0 ml
Distilled water to 100 ml
Filter sterilize and freeze in plasticware.
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