CR1 Soil

The CR1 soil is treated in batches by placing in a heat-resistant pan lined with aluminum foil. Fill the soil to a so depth of one-quarter inch and bake at 150 C for 2 h. Cool and cover the pan with aluminum foil and store in darkness at room temperature. Avoid excessive moisture during storage.

CR1 soil is not a well-defined medium, yet not all soil is suitable for culturing a broad range of algae. Soil used for CR1 originally came from the floodplain of the upper Rio de Flag, Flagstaff, Coconino Co., AZ. It is relatively low in organic content, pH circumneutral and is finely particulate in nature, consisting primarily of volcanic ash and weathered cinders and apparently contains a sufficient array of required trace elements and macronutrients.

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