BG-11 Medium for Blue Green Algae

ATCC Medium 616

NaNO3 1.5 g
K2HPO4 0.04 g
MgSO4 7H2O 0.075 g
CaCl2 2H2O 0.036 g
Citric acid 0.006 g
Ferric ammonium citrate 0.006 g
EDTA (disodium salt) 0.001 g
Na2CO3 0.02 g
Trace metal mix A5 1.0 ml
Agar (if needed) 10.0 g
Distilled water 1.0 L

The pH should be 7.1 after sterilization

Trace metal mix A5:
H3BO3 2.86 g
MnCl2 4H2O 1.81 g
ZnSO4 7H2O 0.222 g
NaMoO4 2H2O 0.39 g
CuSO4 5H2O 0.079 g
Co(NO3)2 6H2O 49.4 mg
Distilled water 1.0 L

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