Ashbey's Nitrogen-free Agar
Agar 15.0 g
Mannitol 15.0 g
CaCl2 2H2O 0.2 g
K2HPO4 0.2 g
MgSO4 7H2O 0.2 g
MoO3 (10% solution) 0.1 ml
FeCl3 (10% solution) 0.05 ml
Distilled water to 1.0 L
pH 7.2 +/- 0.2 at room temperature
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