ASP 2 Medium

Na₂EDTA (3.0 g/L) 10 ml
P1 metals 1.0 ml
Iron solution 1.0 ml
KCl (60 g/L) 10 ml
CaCl₂ 2H₂O (37 g/L) 10 ml
K₂HPO₄ (50 g/L) 1.0 ml
NaCl 18 g
Buffer solution 10 ml
MgSO₄ 2.44 g
Deionized water to 1.0 L

Iron solution:
FeCl₃ 6H₂O 1.95 g
10 M HCl 5 ml
Deionized water to 500 ml

Filter sterilize.

Buffer solution:
TAPS 100 g
TAPSO 100 g
Deionized water to 1.0 L

pH 7.7

P1 metals:
H₃BO₃ 17.15 g
MnCl₂ 4H₂O 2.16 g
ZnSO₄ 7H₂O 0.334 g
CuSO₄ 5H₂O (0.1 g/5 ml)* 0.075 ml
CoCl₂ 6H₂O (0.1 g/5 ml)* 0.3 ml
Na₂MoO₄ 2H₂O (0.11 g/5 ml)* 1.0 ml
Deionized water to 500 ml

*make these stocks fresh and discard after use.

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