Allen and Arnon Medium plus Nitrate

ATCC Medium 1142

Solution A 25.0 ml
Solution B 6.25 ml
KNO3 0.253 g
NaNO3 0.212 g
Purified agar (if needed) 10.0 g
Distilled water 969.0 ml

Add Solution B aseptically after sterilization.

Solution A (A & A minus Phosphate concentrate):
4% MgSO4 7H2O 500.0 ml
1.2% CaCl2 2H2O 500.0 ml
3.8% NaCl 500.0 ml
Microelements stock solution 500.0 ml

Solution B (K2HPO2 Stock solution):
K2HPO2 28.0 g
Distilled water 500.0 ml

Microelements stock solution:
A & A FeEDTA solution 160.0 ml
MnCl2 4H2O 360 mg
MoO3 36.0 mg
ZnSO2 7H2O 44.0 mg
CuSO2 5H2O 15.8 mg
H3BO3 572.0 mg
NH4VO3 4.6 mg
CoCl2 6H2O 8.0 mg
Distilled water 1090.0 ml

A & A FeEDTA solution:
Dissolve 5.2 g KOH in 186 ml distilled water
Add 20.4 g Na2EDTA 2H2O
Dissolve 13.7 g FeSO4 7H2O in 364 ml distilled water
Mix these two solutions
Bubble filtered air through solution until color changes (minutes to hours)
Final pH of FeEDTA solution approximately 3.5

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