Chloroflexus Broth

Na2S0.5 g
Nitrilotriacetic acid0.1 g
Micronutrient solution1.0 ml
FeCl3 solution1.0 ml
CaSO4·2H2O0.06 g
MgSO4·7H2O0.10 g
NaCl0.008 g
KNO30.103 g
NaNO30.689 g
Na2HPO40.111 g
NH4Cl0.2 g
Yeast extract0.5 g
Glycl-glycine0.5 g
Distilled water1.0 L
Prepare above medium and adjust pH to 8.2-8.4. Add 0.5 g Na sulfide. Readjust pH to 8.2-8.4. Filter-sterilize.

FeCl3 Solution:
FeCl30.29 g
Distilled water1.0 L

Micronutrient solution:
H2SO4 (conc.)0.5 ml
MnSO4·7H2O2.28 g
ZnSO4·7H2O0.50 g
H3BO30.50 g
CuSO4·2H2O0.025 g
Na2MoO4·2H2O0.025 g
CoCl2·6H2O0.045 g
Water1.0 L

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