Allen Medium

HEPES buffer2.3 g
NaNO31.5 g
P-IV metal solution1 ml
K2HPO4·7H2O (6 g/L)5 ml
MgSO4·7H2O (6 g/L)5 ml
Na2CO3 (4 g/L)5 ml
CaCl2·2H2O (2.5 g/L)10 ml
Na2SiO3·9H2O (4.64 g/L)10 ml
Citric acid·H2O (4.8 g/L)1 ml
Deionized water to200 ml

pH 7.8

P-IV metal solution:
Na2EDTA·2H2O0.75 g
FeCl3·6H2O0.097 g
MnCl2·4H2O0.041 g
ZnCl20.005 g
CoCl2·6H2O0.002 g
Na2MoO4·2H2O0.004 g
Deionized water to1 L

To approximately 950 ml of dH2O, add ingredients in order listed stirring continuously. The EDTA should be fully dissoled before adding other components. Bring to 1 L with dH2O and store at 4 C.

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