Castenholtz D Medium Modified

Halophilic cyanobacteria

NaCl160.0 g
NaNO30.69 g
Na2HPO40.111 g
KNO30.103 g
MgSO4·7H2O0.1 g
Nitrilotriacetic acid0.1 g
CaSO4·2H2O0.06 g
FeCl30.3 mg
Castenholtz trace metal solution1.0 ml
pH 7.5±0.2 at room temperature

Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.

Castenholtz trace metal solution:
MnSO4·H2O2.28 g
H3BO30.5 g
ZnSO4·7H2O0.5 g
Co(NO3)2·6H2O0.025 g
CuSO4·5H2O0.025 g
Na2MoO4·2H2O0.025 g
H2SO40.5 ml
Distilled water to1.0 L

Back to Media Top page

Back to Cyanosite Homepage