Castenholtz DGN Medium

Glycyl-glycine buffer0.8 g
NaNO30.7 g
NH4Cl0.2 g
Na2HPO40.11 g
KNO30.10 g
MgSO4·7H2O0.10 g
Nitrilotriacetic acid0.10 g
CaSO4·2H2O0.06 g
NaCl 8.0 mg
FeCl3 solution1.0 ml
Castenholtz micronutrient solution0.5 ml
Distilled water to 1.0 L
pH 7.5±0.2 at room temperature

Add nitrilotriacetic acid to 500.0 ml distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components and mix. Adjust pH to 7.5. Bring volume to 1.0 L with distilled water.

FeCl3 solution:
FeCl3·6H2O2.28 g
Distilled water to1.0 L

Castenholtz micronutrient solution:
MnSO4·H2O2.28 g
H3BO30.5 g
ZnSO4·7H2O0.5 g
CoCl2·6H2O0.025 g
CuSO4·5H2O0.025 g
Na2MoO4·2H2O0.025 g
H2SO40.5 ml
Distilled water to1.0 L

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