Allen and Arnon Medium plus Nitrate

ATCC Medium 1142

Solution A25.0 ml
Solution B6.25 ml
KNO30.253 g
NaNO30.212 g
Purified agar (if needed)10.0 g
Distilled water969.0 ml
Add Solution B aseptically after sterilization.

Solution A (A & A minus Phosphate concentrate):
4% MgSO4·7H2O500.0 ml
1.2% CaCl2·2H2O500.0 ml
3.8% NaCl500.0 ml
Microelements stock solution500.0 ml

Solution B (K2HPO2 Stock solution):
K2HPO228.0 g
Distilled water500.0 ml

Microelements stock solution:
A & A FeEDTA solution160.0 ml
MnCl2·4H2O360 mg
MoO336.0 mg
ZnSO2·7H2O44.0 mg
CuSO2·5H2O15.8 mg
H3BO3572.0 mg
NH4VO34.6 mg
CoCl2·6H2O8.0 mg
Distilled water1090.0 ml

A & A FeEDTA solution:
Dissolve 5.2 g KOH in 186 ml distilled water
Add 20.4 g Na2EDTA·2H2O
Dissolve 13.7 g FeSO4·7H2O in 364 ml distilled water
Mix these two solutions
Bubble filtered air through solution until color changes (minutes to hours)
Final pH of FeEDTA solution approximately 3.5

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