S77 plus Vitamins Medium


NaCl 16.00 g
MgSO4 7H2O 2.50 g
CaSO4 2H2O 0.50 g
KCl 0.40 g
Tris 0.05 g
Glycine 0.25 g
KNO3 0.10 g
K2HPO4 0.01 g
KBr 32.50 mg
SrCl2 6H2O 6.50 mg
AlCl4 6H2O 250.00 g
RbCl 100.00 g
LiCl H2O 50.00 g
KI 25.00 g
EDTANa2 50.00 mg
FeSO4 7H2O 2.50 mg
MnSO4 4H2O 203.00 g
ZnSO4 7H2O 22.00 g
CuSO4 5H2O 19.60 g
CoSO4 7H2O 2.38 g
NaMoO4 2H2O 1.26 g
Cyanocobalamin 100.00 ng
Distilled water to 1.0 L

Adjust pH to 4.0 - 5.0. Add 0.10g/L NaSiO3 5H2O while stirring continuously. Adjust pH to 8.0 when the silicate has dissolved.


S88 + vitamins:
Prepare as for
S77 + vitamins, but with the following modifications:

Omit Tris and replace with 0.50 g/L glycylglycine

Add 50.0 g/L thiamine HCl in addition to cyanocobalamin

Omit NaSiO3 5H2O and the associated prior step of adjusting the pH to 4.0 - 5.0



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